Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Single-Strand DNA Binding Proteins

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Silk Fibroin Aggregates at the Air-Water Interface: Amyloid-like Fibrils vs. Self-Assembled Networks.

International journal of molecular sciences·2026

Same author

Structure evolution and high-temperature luminescence versus negative thermal expansion of Ho,Tm-doped Yb<sub>2</sub>Mo<sub>3</sub>O<sub>12</sub> crystals.

Acta crystallographica Section B, Structural science, crystal engineering and materials·2026

Same author

Surface Properties of Recombinant Pea Vicilin and Cupin-1.2 Solutions in 8M Urea.

Polymers·2025

Same author

The Impact of PEO and PVP Additives on the Structure and Properties of Silk Fibroin Adsorption Layers.

Polymers·2025

Same author

Dynamic Properties of Mixed Layers of a Lipid with a Protein or Its Fibrils.

Langmuir : the ACS journal of surfaces and colloids·2025

Same author

Dynamic Properties of β-Casein Fibril Adsorption Layers at the Air-Water Interface.

Polymers·2025

Search research articles

Related Experiment Video

Updated: Mar 9, 2026

Orientational Transition in a Liquid Crystal Triggered by the Thermodynamic Growth of Interfacial Wetting Sheets

Orientational Transition in a Liquid Crystal Triggered by the Thermodynamic Growth of Interfacial Wetting Sheets

Published on: May 15, 2017

Phase Transitions in DNA/Surfactant Adsorption Layers.

Vanda V Lyadinskaya¹, Shi-Yow Lin¹, Alexander V Michailov²

¹National Taiwan University of Science and Technology , Chemical Engineering Department, 43 Keelung Road, Section 4, 106 Taipei, Taiwan.

Langmuir : the ACS Journal of Surfaces and Colloids

|December 21, 2016

Summary

DNA and oppositely charged surfactants dodecyltrimethylammonium bromide (DTAB) and cetyltrimethylammonium bromide (CTAB) form distinct surface phases. This study reveals a first-order phase transition in these adsorption layers, detailing four formation steps.

More Related Videos

Interfacial Molecular-level Structures of Polymers and Biomacromolecules Revealed via Sum Frequency Generation Vibrational Spectroscopy

Interfacial Molecular-level Structures of Polymers and Biomacromolecules Revealed via Sum Frequency Generation Vibrational Spectroscopy

Published on: August 13, 2019

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

Related Experiment Videos

Last Updated: Mar 9, 2026

Orientational Transition in a Liquid Crystal Triggered by the Thermodynamic Growth of Interfacial Wetting Sheets

Orientational Transition in a Liquid Crystal Triggered by the Thermodynamic Growth of Interfacial Wetting Sheets

Published on: May 15, 2017

Interfacial Molecular-level Structures of Polymers and Biomacromolecules Revealed via Sum Frequency Generation Vibrational Spectroscopy

Interfacial Molecular-level Structures of Polymers and Biomacromolecules Revealed via Sum Frequency Generation Vibrational Spectroscopy

Published on: August 13, 2019

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

Area of Science:

Biophysical Chemistry
Surface Science
Materials Science

Background:

DNA-surfactant interactions are crucial for understanding biological systems and developing novel materials.
Complex formation at interfaces influences surface properties and material self-assembly.
Investigating adsorption layers provides insights into molecular interactions and phase behavior.

Purpose of the Study:

To investigate the adsorption layers formed by DNA with dodecyltrimethylammonium bromide (DTAB) and cetyltrimethylammonium bromide (CTAB) at the solution/air interface.
To elucidate the structure of coexisting surface phases and identify the steps of adsorption layer formation.
To characterize the phase transition behavior in DNA-surfactant adsorption layers.

Main Methods:

Surface tensiometry
Dilational surface rheology
Atomic force microscopy (AFM)
Brewster angle microscopy (BAM)
Infrared absorption-reflection spectroscopy (IR-RAS)
Ellipsometry
Adsorption kinetics calculations

Main Results:

Identified time intervals corresponding to the coexistence of two-dimensional phases in DNA-surfactant adsorption layers.
Observed a first-order phase transition, distinct from microaggregate formation in synthetic polyelectrolyte/surfactant systems.
Elucidated the structure of coexisting surface phases using a multi-technique approach.
Distinguished four main steps in the formation of adsorption layers at the DNA/surfactant solution surface.

Conclusions:

DNA-surfactant complexes form distinct coexisting surface phases at the solution/air interface.
The observed phase transition is a first-order process, indicating significant structural rearrangements.
A comprehensive understanding of DNA-surfactant adsorption layer formation was achieved through a multi-technique investigation.