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Related Concept Videos

Size-Exclusion Chromatography01:08

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In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
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Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
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Principles Of Column Chromatography01:13

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The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
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High-Performance Liquid Chromatography: Introduction01:11

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
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Types Of Column Chromatography01:29

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The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
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Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity
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Size-exclusion chromatography using core-shell particles.

Bob W J Pirok1, Pascal Breuer2, Serafine J M Hoppe2

  • 1University of Amsterdam, van't Hoff Institute for Molecular Sciences, Analytical-Chemistry Group, Science Park 904, 1098 XH Amsterdam, The Netherlands; TI-COAST, Science Park 904, 1098 XH Amsterdam, The Netherlands.

Journal of Chromatography. A
|December 21, 2016
PubMed
Summary
This summary is machine-generated.

Core-shell particles offer faster and more resolved separations in size-exclusion chromatography (SEC). This advancement enables quicker molar-mass distribution analysis, crucial for high-molecular-weight analytes.

Keywords:
Core-shell particlesPacking resolution factorSecSize-exclusion chromatographySpecific resolutionUhplc

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Area of Science:

  • Analytical Chemistry
  • Chromatography
  • Separation Science

Background:

  • Size-exclusion chromatography (SEC) is vital for separating high-molecular-weight compounds and determining molar-mass distributions.
  • Achieving very short analysis times is essential for SEC's application in comprehensive two-dimensional liquid chromatography.
  • Core-shell packing materials, known for high efficiency in liquid chromatography, have not been utilized in SEC due to concerns about reduced pore volume.

Purpose of the Study:

  • To investigate the feasibility of using core-shell particles in size-exclusion chromatography (SEC).
  • To compare the performance of contemporary core-shell materials with conventional packing materials for SEC applications.
  • To assess the impact of core-shell particles on resolution and analysis time in SEC.

Main Methods:

  • Evaluation of core-shell particles as stationary phases for size-exclusion chromatography.
  • Comparison of SEC columns packed with core-shell particles against those packed with conventional materials.
  • Analysis of resolution and separation times across different molar-mass ranges.

Main Results:

  • Columns packed with very small core-shell particles demonstrated excellent resolution within specific molar-mass ranges, dependent on pore size.
  • Analysis times using core-shell SEC columns were approximately ten times shorter compared to conventional SEC columns.
  • Core-shell particles show promise for significantly accelerating SEC analyses without compromising resolution.

Conclusions:

  • Core-shell particles are feasible for size-exclusion chromatography, offering enhanced resolution and significantly reduced analysis times.
  • This development opens new possibilities for SEC, particularly in demanding applications like comprehensive two-dimensional liquid chromatography.
  • The use of core-shell materials represents a significant advancement in SEC technology for efficient separation and molar-mass determination.