Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Photosynthesis-driven interactions in the phycosphere enhance bacterial extracellular superoxide production.

The ISME journal·2026
Same author

Toward <i>in situ</i> characterization for advancing membrane performance in water treatment processes.

Fundamental research·2026
Same author

Carbon redistribution driven by autochthonous processes in the world's largest water transfer project: Disrupting natural carbon boundaries.

Water research·2026
Same author

Training gaps and needs of pre-hospital emergency staff in Chengdu: a city-wide cross-sectional study.

Frontiers in medicine·2026
Same author

Computationally intelligent calibration framework for durable soft strain sensors.

Nature communications·2026
Same author

Structural and thermodynamic analyses of a novel β-1,2-glucan binding mode in the ABC transporter solute-binding protein Chy400_4166 from Chloroflexus aurantiacus.

The FEBS journal·2026
Same journal

Corrigendum to "Senescent endothelial cells' response to the degradation of bioresorbable scaffold induces intimal dysfunction accelerating in-stent restenosis" [Acta Biomaterialia 166 (2023) 266-277].

Acta biomaterialia·2026
Same journal

Colorectum and embedded networks of nerve fibers present auxetic responses during uniaxial circumferential extension.

Acta biomaterialia·2026
Same journal

Music-inspired acoustic-piezoelectric stimulation accelerates extracellular vesicle production and programs therapeutic function.

Acta biomaterialia·2026
Same journal

Mutant superoxide dismutase 1-catalyzed hydrogen therapy for amyotrophic lateral sclerosis achieved by intercepting oxidative stress-neuroinflammation crosstalk.

Acta biomaterialia·2026
Same journal

Injectable pH-responsive gelatin methacryloyl hydrogel for cuproptosis-synergized sunitinib therapy and immune reprogramming in clear cell renal cell carcinoma.

Acta biomaterialia·2026
Same journal

Corrigendum to "Injectable hydrogel-assisted local lipopolysaccharide delivery improves immune checkpoint blockade therapy" [Acta Biomaterialia 2025, 194, 153-168].

Acta biomaterialia·2026
See all related articles

Related Experiment Video

Updated: Mar 9, 2026

3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening
11:28

3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening

Published on: October 4, 2017

10.8K

Three-dimensional hepatic lobule-like tissue constructs using cell-microcapsule technology.

Zeyang Liu1, Masaru Takeuchi1, Masahiro Nakajima1

  • 1Department of Micro-Nano Systems Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.

Acta Biomaterialia
|December 21, 2016
PubMed
Summary
This summary is machine-generated.

Researchers developed hepatic lobule-shaped microtissues (HLSM) using cell-microcapsule technology. These HLSM exhibit enhanced liver function compared to spheroids, offering a new building block for artificial livers and implants.

Keywords:
Hepatic lobuleLiver bio-fabricationMicrocapsulePLL-alginateTissue engineering

More Related Videos

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
08:50

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

1.0K
Fabrication of Inverted Colloidal Crystal Polyethylene glycol Scaffold: A Three-dimensional Cell Culture Platform for Liver Tissue Engineering
10:18

Fabrication of Inverted Colloidal Crystal Polyethylene glycol Scaffold: A Three-dimensional Cell Culture Platform for Liver Tissue Engineering

Published on: August 27, 2016

10.4K

Related Experiment Videos

Last Updated: Mar 9, 2026

3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening
11:28

3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening

Published on: October 4, 2017

10.8K
In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
08:50

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

1.0K
Fabrication of Inverted Colloidal Crystal Polyethylene glycol Scaffold: A Three-dimensional Cell Culture Platform for Liver Tissue Engineering
10:18

Fabrication of Inverted Colloidal Crystal Polyethylene glycol Scaffold: A Three-dimensional Cell Culture Platform for Liver Tissue Engineering

Published on: August 27, 2016

10.4K

Area of Science:

  • Biotechnology
  • Tissue Engineering
  • Hepatology

Background:

  • Proper liver function relies on organized hepatocytes.
  • Controlling 3D cellular construct shape is crucial for artificial liver development.
  • Existing methods like spheroids lack precise microscale shape control.

Purpose of the Study:

  • To fabricate hepatic lobule-shaped microtissues (HLSM) for improved in vitro liver models.
  • To assess the hepatic function and structural integrity of HLSM.
  • To demonstrate the assembly of HLSM into a multi-layered hepatic model.

Main Methods:

  • Encapsulation of rat liver (RLC-18) cells within poly-l-lysine-alginate microcapsules.
  • Long-term cultivation (14 days) to promote cell self-assembly into HLSM.
  • Micromanipulation techniques for assembling HLSM into a 3D hepatic lobule model.

Main Results:

  • RLC-18 cells self-assembled into HLSM, fully occupying microcapsules after cultivation.
  • HLSM demonstrated superior hepatic function compared to conventional cell spheroids.
  • A 3D four-layered hepatic lobule model was successfully assembled using HLSM.

Conclusions:

  • Cell-microcapsule technology enables precise 3D shape control for biofunctional hepatic tissues.
  • HLSM serve as advanced tissue-engineered building blocks for larger constructs and potential clinical applications.
  • This technology advances the development of artificial organs and cellular implants.