Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Yeast Signaling01:28

Yeast Signaling

18.3K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
18.3K
Protein Networks02:26

Protein Networks

4.6K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The RNA-bound proteome of MRSA reveals post-transcriptional roles for helix-turn-helix DNA-binding and Rossmann-fold proteins.

Nature communications·2022
Same author

Bacterial RNA in extracellular vesicles: A new regulator of host-pathogen interactions?

Biochimica et biophysica acta. Gene regulatory mechanisms·2020
Same author

Organic phase separation opens up new opportunities to interrogate the RNA-binding proteome.

Current opinion in chemical biology·2020
Same author

Probing the RNA-Binding Proteome from Yeast to Man: Major Advances and Challenges.

Methods in molecular biology (Clifton, N.J.)·2019
Same author

Fast and unbiased purification of RNA-protein complexes after UV cross-linking.

Methods (San Diego, Calif.)·2019
Same author

TriPepSVM: de novo prediction of RNA-binding proteins based on short amino acid motifs.

Nucleic acids research·2019

Related Experiment Video

Updated: Mar 9, 2026

mRNA Interactome Capture from Plant Protoplasts
12:29

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

9.6K

RNA interactome capture in yeast.

Benedikt M Beckmann1

  • 1European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117 Heidelberg, Germany; IRI for Life Sciences & Institut für Biologie, Humboldt-Universität zu Berlin, Philippstrasse 13, 10115 Berlin, Germany.

Methods (San Diego, Calif.)
|December 21, 2016
PubMed
Summary
This summary is machine-generated.

We developed a new method to identify RNA-binding proteins (RBPs) in yeast. This protocol uses photoactivatable-ribonucleoside-enhanced crosslinking (PAR-CL) for unbiased RBP discovery.

Keywords:
RBPRNA labelingUV crosslinkingYeastmRNA

More Related Videos

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions
14:23

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

Published on: June 6, 2018

14.3K
Split-Ubiquitin Based Membrane Yeast Two-Hybrid MYTH System: A Powerful Tool For Identifying Protein-Protein Interactions
14:04

Split-Ubiquitin Based Membrane Yeast Two-Hybrid MYTH System: A Powerful Tool For Identifying Protein-Protein Interactions

Published on: February 1, 2010

32.1K

Related Experiment Videos

Last Updated: Mar 9, 2026

mRNA Interactome Capture from Plant Protoplasts
12:29

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

9.6K
A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions
14:23

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

Published on: June 6, 2018

14.3K
Split-Ubiquitin Based Membrane Yeast Two-Hybrid MYTH System: A Powerful Tool For Identifying Protein-Protein Interactions
14:04

Split-Ubiquitin Based Membrane Yeast Two-Hybrid MYTH System: A Powerful Tool For Identifying Protein-Protein Interactions

Published on: February 1, 2010

32.1K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • RNA-binding proteins (RBPs) are crucial for post-transcriptional gene regulation in eukaryotes.
  • Identifying RBPs is essential for understanding gene expression control.

Purpose of the Study:

  • To develop an unbiased method for identifying RNA-binding proteins in Saccharomyces cerevisiae.
  • To present a detailed protocol for yeast RNA interactome capture.

Main Methods:

  • The study utilized a yeast RNA interactome capture protocol.
  • Key techniques include RNA labeling, photoactivatable-ribonucleoside-enhanced crosslinking (PAR-CL) using 365nm UV light, and purification of protein-bound mRNA.
  • The protocol is designed for common laboratory workflows and takes approximately 3 days.

Main Results:

  • The developed protocol allows for the unbiased identification of RBPs in yeast.
  • The study provides a comprehensive explanation of the method's steps.
  • Findings regarding the choice of crosslinking method in yeast were discussed.

Conclusions:

  • The described yeast RNA interactome capture protocol is a valuable tool for RBP discovery.
  • The method is practical, reproducible, and aids in understanding RNA-protein interactions.
  • Further insights into crosslinking strategies for yeast were provided.