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Surface acoustic wave enabled pipette on a chip.

Muhsincan Sesen1, Citsabehsan Devendran1, Sean Malikides1

  • 1Laboratory for Microsystems, Department of Mechanical and Aerospace Engineering, Monash University, Clayton, VIC 3800, Australia. adrian.neild@monash.edu.

Lab on a Chip
|December 21, 2016
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Summary
This summary is machine-generated.

This study introduces a programmable microfluidic chip for precise sample pipetting from droplets. The Pipette on a chip (PoaCH) system uses surface acoustic waves for accurate, non-contact liquid handling in microfluidics.

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Area of Science:

  • Microfluidics and Lab-on-a-Chip Technologies
  • Acoustic Manipulation and Droplet Science

Background:

  • Microfluidic devices enable rapid, high-throughput production of pico- to nanoliter droplets for chemical and biological studies.
  • Current methods lack precise control over sample distribution and quantity on-chip, hindering replication of micro well plate-based screening.
  • There is a need for accurate, programmable sample handling within microfluidic systems.

Purpose of the Study:

  • To develop a programmable microfluidic chip for accurate, non-contact sample pipetting from mobile droplets.
  • To enable precise control over sample volume and location for high-throughput screening applications at reduced scales.

Main Methods:

  • Development of a 'Pipette on a chip' (PoaCH) system utilizing surface acoustic waves (SAWs).
  • Non-contact ejection of partial droplets from mobile sources into customizable reaction chambers.
  • Demonstration of programmable droplet manipulation within closed microchannels.

Main Results:

  • Achieved droplet pipetting volumes ranging from 150 pL to 850 pL.
  • Demonstrated high precision in droplet volume control, down to tens of picoliters.
  • Verified robust and contamination-free droplet manipulation.

Conclusions:

  • The PoaCH system offers a programmable solution for accurate sample pipetting in microfluidics.
  • Enables precise, non-contact droplet manipulation, crucial for advanced screening applications.
  • Facilitates integration with existing lab-on-a-chip systems for enhanced functionality.