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PRKAR1A is a functional tumor suppressor inhibiting ERK/Snail/E-cadherin pathway in lung adenocarcinoma

Shaoqiang Wang1, Yuanda Cheng1, Yingying Zheng2

  • 1Department of Thoracic Surgery, Xiangya Hospital, Central South University, Xiangya Road 87th, Changsha, 410008, Hunan, P. R. China.

Scientific Reports
|December 21, 2016
PubMed

Insights

Protein Kinase cAMP-Dependent Regulatory Type I Alpha (PRKAR1A) acts as a tumor suppressor in lung adenocarcinoma. Downregulation of PRKAR1A promotes tumor progression and metastasis by activating the ERK/Snail/E-cadherin pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Protein Kinase cAMP-Dependent Regulatory Type I Alpha (PRKAR1A) is implicated in various tumors.
  • PRKAR1A downregulation was previously observed in lung adenocarcinoma.
  • Its precise role as a tumor suppressor in this context requires further elucidation.

Purpose of the Study:

  • To investigate the clinical significance of PRKAR1A in lung adenocarcinoma.
  • To determine the biological function of PRKAR1A as a tumor suppressor.
  • To elucidate the molecular mechanisms underlying PRKAR1A's function.

Main Methods:

  • Correlation analysis of PRKAR1A transcript levels with clinical data.
  • In vitro studies using cell lines (H1299, A549) with PRKAR1A re-expression or knockdown.
  • In vivo metastasis models in nude mice.
  • Western blot analysis to assess protein expression and signaling pathways (ERK/Snail/E-cadherin).

Main Results:

  • Low PRKAR1A transcript levels correlate with tumor progression and reduced overall survival.
  • PRKAR1A re-expression suppressed proliferation and migration; PRKAR1A knockdown enhanced these processes in vitro and in vivo.
  • PRKAR1A knockdown promoted lung colonization of circulating tumor cells.
  • PRKAR1A inhibits E-cadherin expression by activating the ERK/Snail signaling pathway.

Conclusions:

  • PRKAR1A functions as a potent tumor suppressor in lung adenocarcinoma.
  • The PRKAR1A-ERK-Snail-E-cadherin axis represents a potential therapeutic target.
  • Targeting this pathway may offer new strategies for lung adenocarcinoma treatment.

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