Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

946
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
946
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

21.6K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
21.6K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

14.9K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
14.9K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.7K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.7K
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

1.8K
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
1.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Esculetin alleviates IgA nephropathy in rats by improving intestinal mucosal function through the IL-17/NF-κB signaling.

Toxicological research·2026
Same author

Concurrent Mantle Cell Lymphoma and Paraneoplastic Membranous Nephropathy.

Kidney medicine·2026
Same author

High-power single-mode master oscillator power amplifier system at 795  nm for a 128-channel SERF magnetometer.

Applied optics·2026
Same author

AI-enhanced Wrist-Hand US Image Acquisition: Development and Initial Clinical Evaluation.

Radiology·2026
Same author

Synergizing high-sugar traits and interspecific diversity: temporal dynamics and trade-offs in temperate grasslands.

Frontiers in plant science·2026
Same author

Astragaloside IV Ameliorates Allergic Rhinitis Comorbid With Asthma by Inhibiting the Antigen-Presenting Function of B Cells.

Basic & clinical pharmacology & toxicology·2026

Related Experiment Video

Updated: Mar 9, 2026

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

10.2K

Deep imaging in scattering media with selective plane illumination microscopy.

Adithya Kumar Pediredla1, Shizheng Zhang1, Ben Avants1

  • 1Rice University, Department of Electrical and Computer Engineering, 6100 Main Street, Houston, Texas 77005, United States.

Journal of Biomedical Optics
|December 21, 2016
PubMed
Summary

Selective plane illumination microscopy (SPIM) can achieve deep tissue imaging comparable to two-photon microscopy (2PM) by minimizing excitation path length, offering a cost-effective alternative for scattering biological tissues.

More Related Videos

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.7K
Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
11:57

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

Published on: May 20, 2013

14.0K

Related Experiment Videos

Last Updated: Mar 9, 2026

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

10.2K
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.7K
Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
11:57

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

Published on: May 20, 2013

14.0K

Area of Science:

  • Biophotonics
  • Optical Imaging
  • Microscopy

Background:

  • Light scattering in biological tissues limits optical imaging depth.
  • Two-photon microscopy (2PM) enables deep tissue imaging but requires expensive, bulky pulsed lasers.
  • Existing single-photon techniques have limited penetration depth in scattering media.

Purpose of the Study:

  • To investigate the potential of selective plane illumination microscopy (SPIM) for deep tissue imaging.
  • To compare the imaging depth of SPIM with 2PM and other single-photon methods in scattering media.
  • To determine if SPIM can be a viable, less costly alternative to 2PM for deep imaging.

Main Methods:

  • Theoretical modeling and experimental validation of SPIM performance.
  • Quantitative assessment of imaging depth in scattering biological tissues.
  • Comparative analysis of SPIM, 2PM, epifluorescence, and confocal microscopy.

Main Results:

  • Minimized excitation path length enables SPIM to image nearly as deep as 2PM.
  • SPIM achieves greater than twice the imaging depth of other single-photon techniques in scattering media.
  • SPIM demonstrates potential for deep imaging without high-powered pulsed lasers.

Conclusions:

  • SPIM, with optimized excitation path length, offers a promising alternative for deep optical imaging in scattering tissues.
  • SPIM provides a cost-effective and potentially portable solution for deep biological imaging compared to 2PM.
  • This study highlights SPIM's capability to overcome depth limitations in scattering media.