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Related Concept Videos

Operons02:09

Operons

55.2K
Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by...
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Inducible Operons: lac Operon01:25

Inducible Operons: lac Operon

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The lac operon in Escherichia coli is a model for understanding inducible gene regulation and metabolic flexibility. It integrates local control by lactose and global regulation through catabolite repression, enabling E. coli to preferentially metabolize glucose when available and switch to lactose utilization when glucose is scarce.Structure and Function of the lac OperonThe lac operon contains three structural genes: lacZ (β-galactosidase), lacY (lactose permease), and lacA...
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Related Experiment Video

Updated: Mar 9, 2026

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
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Development of a Transcription Factor-Based Lactam Biosensor.

Jingwei Zhang1, Jesus F Barajas1, Mehmet Burdu1

  • 1Joint BioEnergy Institute , Emeryville, California United States.

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|December 21, 2016
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Summary

Researchers developed a novel microbial biosensor for detecting lactams, crucial chemicals for nylon production. This biosensor enables high-throughput screening for improved biological lactam manufacturing.

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Area of Science:

  • Biotechnology and Metabolic Engineering
  • Chemical Biology
  • Chemoinformatics

Background:

  • Lactams are vital commodity chemicals, with millions of tons produced annually for nylon manufacturing.
  • Current biological production methods for lactams require enhancement through high-throughput sensing technologies.
  • Development of specific biosensors is critical for advancing metabolic engineering efforts in lactam biosynthesis.

Purpose of the Study:

  • To identify and characterize novel microbial biosensors for lactam detection.
  • To enable high-throughput screening for improved lactam biosynthesis.
  • To apply a chemoinformatic approach for biosensor discovery.

Main Methods:

  • Utilized a chemoinformatic strategy termed analogue generation toward catabolizable chemicals (AGTC), inspired by small molecule drug discovery.
  • Screened for biosensors using the ChnR/Pb transcription factor-promoter pair.
  • Validated the sensing capabilities and specificity of the identified lactam biosensor.

Main Results:

  • Discovered a novel microbial biosensor based on the ChnR/Pb transcription factor-promoter system.
  • The biosensor effectively detects ε-caprolactam, δ-valerolactam, and butyrolactam in a dose-dependent manner.
  • Demonstrated high specificity, distinguishing lactams from their biosynthetic intermediates.

Conclusions:

  • The developed ChnR/Pb-based biosensor is a promising tool for detecting multiple lactams.
  • This biosensor facilitates high-throughput screening for metabolic engineering of lactam production.
  • Potential applications include optimizing industrial-scale, high-titer lactam biosynthesis.