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SPR platform based on image acquisition for HER2 antigen detection.

Johny P Monteiro¹, Sheila M Predabon¹, Elton G Bonafé²

¹Materials Chemistry and Sensors Laboratories, Department of Chemistry, State University of Maringá, Colombo Avenue 5790, 87020-900, Maringá, PR, Brazil.

|December 21, 2016

Summary

This summary is machine-generated.

A novel surface plasmon resonance (SPR) biosensor utilizing nanohole arrays offers a label-free method for detecting HER2 antigen, crucial for breast cancer diagnosis and prognosis. This advanced biosensor overcomes limitations of traditional assays, enabling direct and sensitive detection of biomarkers.

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Area of Science:

Biomedical Engineering
Nanotechnology
Analytical Chemistry

Background:

HER2 antigen is a critical biomarker for breast cancer diagnosis and prevention.
Traditional enzyme-linked immunosorbent assays (ELISA) for HER2 detection have limitations, including suppressed thermodynamic-kinetic studies and potential false positives from labeled molecules.
Surface plasmon resonance (SPR) biosensors offer sensitive, label-free detection, overcoming conventional immunoassay drawbacks.

Purpose of the Study:

To develop and evaluate a new SPR biosensor for sensitive HER2 antigen assessment.
To overcome the limitations of traditional immunoassays for HER2 determination.
To demonstrate the feasibility of the SPR biosensor for direct biomarker detection in breast cancer diagnostics.

Main Methods:

Fabrication of an SPR biosensor using nanohole arrays on a gold thin film.
Signal transduction via transmitted light measurements from array image acquisitions.
Detection of HER2 antigen using a microfluidic system for sample delivery.

Main Results:

The SPR biosensor demonstrated high sensitivity to refractive index variations (4146 intensity units/RIU).
Efficient detection of a known HER2 antigen concentration (3 ng/mL) from aqueous solutions was achieved.
The device proved feasible for direct biomolecular assessment without labeled molecules.

Conclusions:

The proposed SPR biosensor is a powerful tool for bioassays, particularly for breast cancer diagnosis and prognosis.
Label-free detection via SPR imaging overcomes limitations of conventional methods.
This technology holds significant potential for advancing sensitive and accurate biomarker detection.