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Biologically active recombinant carp LH as a spawning-inducing agent for carp.
Joseph Aizen1, Lian Hollander-Cohen1, Michal Shpilman1
1The Robert H. Smith Faculty of AgricultureFood and Environment, Department of Animal Sciences, The Hebrew University of Jerusalem, Rehovot, Israel.
The Journal of Endocrinology
|December 22, 2016
Summary
Researchers developed a recombinant carp luteinizing hormone (rcLh) as a potential alternative for fish spawning induction. This novel agent demonstrated effectiveness comparable to carp pituitary extract (CPE), enhancing hormone secretion and improving spawning success and fertilization rates in carp.
Area of Science:
- Aquaculture and Reproductive Biology
- Biotechnology and Recombinant Protein Production
Background:
- Current methods for inducing spawning in carp rely on carp pituitary extract (CPE) or synthetic hormone combinations.
- There is a need for scalable and effective alternatives to traditional spawning induction agents in aquaculture.
Purpose of the Study:
- To produce large-scale recombinant carp luteinizing hormone (rcLh) as a potential alternative for spawning induction.
- To evaluate the efficacy of rcLh in stimulating reproductive hormone secretion and inducing ovulation and spawning in carp.
Main Methods:
- Production of a single-chain rcLh using the methylotrophic yeast Pichia pastoris.
- In vivo testing of rcLh's ability to stimulate estradiol (E2) and 17α,20β-dihydroxy-4-pregnen-3-one (DHP) secretion.
- Assessment of rcLh's potency in inducing ovulation and spawning, comparing its effectiveness to CPE.
Main Results:
- rcLh significantly enhanced E2 and DHP secretion in a dose-dependent manner, similar to CPE.
- DHP secretion increased approximately 5 hours before spawning following rcLh administration.
- At the highest dose, rcLh surpassed CPE in spawning success and fertilization rates.
Conclusions:
- Recombinant carp luteinizing hormone (rcLh) can effectively elicit in vivo DHP secretion and trigger spawning in carp.
- rcLh shows significant potential as a superior alternative to CPE for inducing spawning in aquaculture.
