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Clinical Application of Targeted Next Generation Sequencing for Colorectal Cancers

Quitterie Fontanges1, Ricardo De Mendonca2, Isabelle Salmon3

  • 1Department of Pathology, Erasme Hospital, Université Libre de Bruxelles, 1070 Brussels, Belgium. quitterie.fontanges@erasme.ulb.ac.be.

International Journal of Molecular Sciences
|December 22, 2016
PubMed

Insights

Next-generation sequencing (NGS) is crucial for molecular profiling in colorectal cancer (CRC). This review highlights NGS

Area of Science:

  • Oncology
  • Genomics
  • Molecular Diagnostics

Background:

  • Personalized medicine and targeted therapies necessitate molecular tumor profiling for colorectal cancer (CRC).
  • RAS (KRAS and NRAS) mutational status is a prerequisite for anti-EGFR therapy in CRC patients.
  • Emerging data on molecular biomarkers emphasize the need for validated, sensitive, and accessible screening tests.

Purpose of the Study:

  • To review the clinical applications of Next-Generation Sequencing (NGS) technology in colorectal cancer.
  • To highlight the role of NGS in molecular profiling for guiding CRC therapy and clinical trials.

Main Methods:

  • Review of existing literature on NGS technology and its application in colorectal cancer.
  • Focus on targeted NGS as a method for parallel sequencing of multiple genetic alterations.
  • Emphasis on cost-effectiveness and minimal DNA input requirements for NGS.

Main Results:

  • NGS is increasingly supplanting other genomic profiling technologies.
  • Targeted NGS enables simultaneous detection of numerous genetic alterations.
  • NGS offers a cost-effective solution for comprehensive molecular screening in CRC.

Conclusions:

  • NGS is a powerful tool for molecular profiling in colorectal cancer.
  • Its application supports the implementation of personalized medicine and multi-modal therapy strategies.
  • NGS facilitates improved clinical trial design and patient stratification.
Keywords:
clinical applicationcolorectal cancertargeted next generation sequencing

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