Effect of grape juice consumption on antioxidant activity and interleukin-6 concentration in lactating rats
Sabrina Baptista Alves Faria1, Vanessa Rosse, Juliana Furtado Dias
1Federal Fluminense University. Rio de Janeiro, Brazil. vilma.blondet@gmail.com.
Objective:
This study evaluated the effect of grape juice consumption on the antioxidant capacity and the interleukin-6 blood level of lactating rats.
Material And Method:
Eighteen Wistar rats, lactating females adult, were divided into two groups: control group (CG) and grape juice group (GJG). The antioxidant activity was determined by the method of 2,2-diphenyl-1-picrylhydrazyl (DPPH)-kidnapping of free radicals (DPPH) and oxygen radical absorbance capacity (ORAC), and the interleukin-6 was determined by Enzyme-Linked Immunosorbent Assay (ELISA) method. The data were presented as mean and standard deviation.
Results:
The antioxidant capacity was higher (p < 0.05) in the GJG (25.00 ± 3.08 µmol eq. Trolox/g) than in the CG (10.00 ± 3.11 µmol eq. Trolox/g), by the ORAC method. The interleukin-6 (pg/ml) level was lower in the grape juice group than in CG.
Conclusion:
The consumption of grape juice during lactation improves the antioxidant capacity in lactating rats and seems capable to decrease the inflammatory activity.
