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Bulk segregant analysis (BSA) for improving cold stress resistance in maize using SSR markers
M Q U Farooqi, K J Sa, T K Hong1
1Department of Applied Plant Sciences, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Korea.
Genetics and Molecular Research : GMR
|December 22, 2016
Summary
Researchers used simple sequence repeat (SSR) markers to analyze genetic diversity in maize lines with varying cold tolerance. This identified specific SSR markers crucial for breeding maize varieties with enhanced cold hardiness.
Area of Science:
- Plant genetics
- Molecular biology
- Agricultural science
Background:
- Cold tolerance is a critical trait for maize (Zea mays L.) production, impacting yield in temperate regions.
- Understanding the genetic basis of cold tolerance is essential for developing improved maize cultivars.
- Simple Sequence Repeat (SSR) markers offer a valuable tool for assessing genetic diversity and identifying trait-linked loci.
Purpose of the Study:
- To investigate genetic diversity and relationships among maize inbred lines with contrasting cold tolerance.
- To identify specific SSR markers associated with high and low cold tolerance in maize.
- To evaluate the potential of identified SSR markers for marker-assisted breeding in maize.
Main Methods:
- Bulk Segregant Analysis (BSA) was employed to study eight inbred maize lines.
- Genetic diversity and relationships were assessed using 100 SSR markers linked to cold tolerance.
- Dendrogram analysis was used to visualize genetic clustering based on SSR marker data.
Main Results:
- A total of 319 alleles were identified, with 128 specific to high cold tolerance and 61 to poor cold tolerance.
- Average gene diversity and polymorphic information content were 0.63 and 0.58, respectively.
- Dendrogram analysis revealed three main clusters, effectively distinguishing high from poorly cold-tolerant lines. Specific SSR markers like bnlg1273 and umc1124 were highlighted for their potential.
Conclusions:
- SSR markers are effective in characterizing genetic diversity related to cold tolerance in maize.
- Identified specific SSR markers can serve as valuable tools for marker-assisted selection in cold-tolerant maize breeding programs.
- This study provides a foundation for future research aimed at enhancing maize cold hardiness through genetic approaches.
