Piper betle Induced Cytoprotective Genes and Proteins via the Nrf2/ARE Pathway in Aging Mice

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Nor Syahida Aliahmat1, Nur Fathiah Abdul Sani, Wan Nuraini Wan Hasan

  • 1Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.

Journal of Nutrigenetics and Nutrigenomics
|December 22, 2016
PubMed
Abstract

Insights

Piper betle extract activates antioxidant defenses in aging rats by upregulating protective enzymes through the Nrf2/ARE pathway. This study reveals Piper betle

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Gerontology

Background:

  • Aging is associated with increased oxidative stress.
  • The nuclear factor erythroid 2-related factor 2 (Nrf2)/ARE pathway is crucial for cellular antioxidant defense.
  • Piper betle (PB) is a plant with potential medicinal properties.

Purpose of the Study:

  • To investigate the antioxidant mechanism of aqueous Piper betle extract in aging rats.
  • To determine if Piper betle influences the Nrf2/ARE signaling pathway.

Main Methods:

  • Analysis of phase II detoxifying and antioxidant enzyme genes and proteins.
  • Utilized QuantiGenePlex 2.0 Assay and Western blot analysis.
  • Investigated the translocation of Nrf2.

Main Results:

  • Piper betle extract significantly induced genes and proteins of phase II and antioxidant enzymes, including NAD(P)H quinone oxidoreductase 1 and catalase.
  • Enzyme expression was stimulated by the translocation of Nrf2 into the nucleus.
  • This indicates the involvement of the ARE (antioxidant response element) in the promoter region of these genes.

Conclusions:

  • Piper betle extract activates cytoprotective genes via the Nrf2/ARE signaling pathway.
  • This study elucidates the antioxidant mechanism of Piper betle in the context of aging.
  • Provides novel insights into the therapeutic potential of Piper betle for age-related oxidative stress.

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