Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers01:19

Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers

681
Cardiac biomarkers are critical in diagnosing, prognosing, and managing cardiovascular diseases. Routine measurement of specific biomarkers such as B-type natriuretic peptide (BNP), C-reactive protein (CRP), and homocysteine (Hcy) is common practice in clinical settings to evaluate heart function and predict cardiovascular events.
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...
681

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of key baseline disease characteristics on aflibercept 8 mg dosing interval extension: A post hoc 96-week analysis of PULSAR.

Ophthalmology. Retina·2026
Same author

Ocular toxoplasmosis in Latin American and European patients: clinical characteristics, visual outcomes, and recurrence patterns.

Journal of ophthalmic inflammation and infection·2026
Same author

Beyond monotherapy in diabetic macular edema: sequential and combination therapy-when and why?

Archivos de la Sociedad Espanola de Oftalmologia·2026
Same author

Peripapillary Choroidal Neovascularization in Adults: Spectrum of Etiologies and Clinical Features.

Investigative ophthalmology & visual science·2026
Same author

I-SCREEN: Development of an AI-based infrastructure for community-wide screening and prediction of progression in age-related macular degeneration providing accessible shared care.

Eye (London, England)·2026
Same author

Vascular endothelial growth factor inhibitors outcomes in good vision eyes with neovascular age-related macular degeneration: Fight Retinal Blindness! SPAIN Report 4.

Canadian journal of ophthalmology. Journal canadien d'ophtalmologie·2026

Related Experiment Video

Updated: Mar 9, 2026

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment
07:26

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment

Published on: July 18, 2017

12.3K

C-reactive protein isoforms differentially affect outer blood-retinal barrier integrity and function.

Blanca Molins1,2, Anna Pascual, Méndez3,4

  • 1Institut d'Investigacions Biomèdiques Agustí Pi i Sunyer, Hospital Clínic de Barcelona, Barcelona, Spain; bmolins@clinic.ub.es.

American Journal of Physiology. Cell Physiology
|December 23, 2016
PubMed
Summary
This summary is machine-generated.

Monomeric C-reactive protein (mCRP) disrupts the retinal barrier, impacting age-related macular degeneration (AMD) progression. Corticosteroids and anti-VEGF drugs show potential in mitigating mCRP-induced damage to the outer blood-retinal barrier (oBRB).

Keywords:
C-reactive proteinblood-retinal barriermacular degenerationtight junctions

More Related Videos

Efficient Dissection and Culture of Primary Mouse Retinal Pigment Epithelial Cells
08:33

Efficient Dissection and Culture of Primary Mouse Retinal Pigment Epithelial Cells

Published on: February 10, 2021

7.3K
An Acute Retinal Model for Evaluating Blood Retinal Barrier Breach and Potential Drugs for Treatment
09:33

An Acute Retinal Model for Evaluating Blood Retinal Barrier Breach and Potential Drugs for Treatment

Published on: September 13, 2016

7.6K

Related Experiment Videos

Last Updated: Mar 9, 2026

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment
07:26

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment

Published on: July 18, 2017

12.3K
Efficient Dissection and Culture of Primary Mouse Retinal Pigment Epithelial Cells
08:33

Efficient Dissection and Culture of Primary Mouse Retinal Pigment Epithelial Cells

Published on: February 10, 2021

7.3K
An Acute Retinal Model for Evaluating Blood Retinal Barrier Breach and Potential Drugs for Treatment
09:33

An Acute Retinal Model for Evaluating Blood Retinal Barrier Breach and Potential Drugs for Treatment

Published on: September 13, 2016

7.6K

Area of Science:

  • Ophthalmology
  • Immunology
  • Cell Biology

Background:

  • The retinal pigment epithelium (RPE) is crucial for the outer blood-retinal barrier (oBRB) and is affected in age-related macular degeneration (AMD).
  • C-reactive protein (CRP) is an inflammation biomarker linked to AMD, existing as monomeric (mCRP) and pentameric (pCRP) isoforms with potentially distinct roles.

Purpose of the Study:

  • To investigate the differential effects of mCRP and pCRP on RPE barrier function.
  • To assess the therapeutic potential of common clinical drugs against mCRP-induced RPE dysfunction.

Main Methods:

  • Utilized RPE cell cultures to examine the impact of mCRP and pCRP on barrier permeability and tight junction proteins (ZO-1, occludin).
  • Evaluated the efficacy of methylprednisolone (corticosteroid) and bevacizumab (anti-VEGF) in preventing or reversing mCRP-induced RPE barrier disruption and IL-8 production.

Main Results:

  • mCRP, but not pCRP, significantly increased RPE paracellular permeability and disrupted ZO-1 and occludin.
  • Both methylprednisolone and bevacizumab effectively prevented mCRP-induced barrier dysfunction and IL-8 elevation in RPE cells.
  • Bevacizumab demonstrated the ability to reverse established mCRP-induced IL-8 increase.

Conclusions:

  • mCRP may compromise the oBRB integrity, contributing to AMD pathogenesis.
  • Corticosteroids and anti-VEGF agents represent potential therapeutic strategies for managing mCRP-mediated RPE damage.