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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Mar 9, 2026

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Published on: March 24, 2023

Flow cytometric data analysis of circulating progenitor cell stability.

Ernestine A Mahar¹, Liping Mou¹, Salim S Hayek¹

¹Emory University School of Medicine, Atlanta, GA, USA.

|December 23, 2016

Summary

Blood progenitor cell stability in stored samples is crucial for research. This study found that circulating progenitor cells (CPC) in blood samples stored at 4°C remain stable for up to 48 hours, ensuring reliable data for analysis.

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Last Updated: Mar 9, 2026

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Published on: February 16, 2024

Area of Science:

Cardiovascular Research
Hematology
Biomedical Science

Background:

Circulating progenitor cells (CPC) are vital for vascular health.
Previous research linked higher CPC levels to impaired coronary flow reserve in women with non-obstructive coronary artery disease.
Accurate quantification of CPC requires stable blood samples.

Purpose of the Study:

To assess the stability of circulating progenitor cells in blood samples stored at 4°C.
To determine the optimal time frame for analyzing progenitor cell content in stored blood.
To ensure reliable data for future cardiovascular research involving CPC.

Main Methods:

Blood samples from healthy volunteers (n=6) were collected and stored at 4°C in EDTA tubes.
Flow cytometry was used to quantify progenitor cell subsets (CD34+ and CD34+/CD133+) at 0-4h, 24h, and 48h post-collection.
Samples were fixed with 1% Paraformaldehyde, and 1,000,000 events were analyzed per sample.

Main Results:

No significant differences were observed in CD34+ cell counts between time points (P=0.68).
No significant differences were observed in CD34+/CD133+ cell counts between time points (P=0.74).
Blood progenitor cell content remained stable for up to 48 hours when stored at 4°C.

Conclusions:

Blood samples can be stored at 4°C for up to 48 hours without compromising the stability of circulating progenitor cells.
This finding supports the feasibility of standardized protocols for CPC analysis in research settings.
Consistent CPC data can be obtained from samples stored under these conditions, facilitating further investigation into their role in cardiovascular disease.