A Ratiomeric Fluorescent Sensor for Zn²⁺ Based on N,N'-Di(quinolin-8-yl)oxalamide

A new ratiometric fluorescent sensor (DQO) based on N,N'-Di(quinolin-8-yl) oxalamide has been designed and synthesized for selective detection of Zn²⁺. The fluorescence ratio (I _{536 nm}/I _{450 nm}) of DQO was enhanced 10-fold when Zn²⁺ was present in a buffer aqueous solution at pH 8.66. The sensor showed linear response toward Zn²⁺ in the concentration range 0-15 μM, and the detection limit was calculated to be 2.4 μM. A Job's plot implied the formation of a DQO/Zn²⁺ complex with 1:1 stoichiometry, and the apparent association constant of DQO/Zn²⁺ complex was computed to be 1.5 × 10⁴ M^-1.