Eco-Friendly Composite of Fe₃O₄-Reduced Graphene Oxide Particles for Efficient Enzyme Immobilization

A novel type of spherical and porous composites were synthesized to dually benefit from reduced graphene oxide (rGO) and magnetic materials as supports for enzyme immobilization. Three magnetic composite particles of Fe₃O₄ and rGO containing 71% (rGO-Fe₃O₄-M1), 36% (rGO-Fe₃O₄-M2), and 18% (rGO-Fe₃O₄-M3) Fe were prepared using a one-pot spray pyrolysis method and were used for the immobilization of the model enzymes, laccase and horseradish peroxidase (HRP). The rGO-Fe₃O₄ composite particles prepared by spray pyrolysis process had a regular shape, finite size, and uniform composition. The immobilization of laccase and HRP on rGO-Fe₃O₄-M1 resulted in 112 and 89.8% immobilization efficiency higher than that of synthesized pure Fe₃O₄ and rGO particles, respectively. The stability of laccase was improved by approximately 15-fold at 25 °C. Furthermore, rGO-Fe₃O₄-M1-immobilized laccase exhibited 92.6% of residual activity after 10 cycles of reuse and was 192% more efficient in oxidizing different phenolic compounds than the free enzyme. Therefore, these unique composite particles containing rGO and Fe₃O₄ may be promising supports for the efficient immobilization of industrially important enzymes with lower acute toxicity toward Vibrio fischeri than commercial pure Fe₃O₄ particles.