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Multimodal Sensing Strategy Using pH Dependent Fluorescence Switchable System.

A Muthurasu1,2, V Ganesh1,2

  • 1Electrodics and Electrocatalysis (EEC) Division, CSIR - Central Electrochemical Research Institute (CSIR - CECRI), Karaikudi - 630003, Tamilnadu, India.

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|December 23, 2016
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Summary
This summary is machine-generated.

This study developed fluorescent gold nanoparticles (FL-Au NPs) for selective cysteine (Cys) detection using a multimodal sensing strategy. The method offers tunable sensitivity for Cys detection across various pH and concentration levels.

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Area of Science:

  • Nanotechnology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Biomolecule-assisted synthesis offers novel routes for nanomaterial preparation.
  • Fluorescent gold nanoparticles (FL-Au NPs) show promise in sensing applications.
  • Selective detection of cysteine (Cys) is crucial in various biological and chemical analyses.

Purpose of the Study:

  • To synthesize FL-Au NPs using glucose oxidase as a reducing and stabilizing agent.
  • To develop a multimodal sensing strategy for selective cysteine detection.
  • To investigate the application of FL-Au NPs in fluorescence turn OFF-ON, naked eye, and electrochemical sensing.

Main Methods:

  • Utilized glucose oxidase enzyme for the reduction and stabilization of gold nanoparticles.
  • Employed a fluorescence turn-OFF strategy based on the interaction between gold nanoparticles and thiols.
  • Developed a fluorescence switch-ON strategy involving pH-dependent fluorescence quenching and recovery.
  • Incorporated naked eye detection through visual color changes.
  • Performed electrochemical sensing of cysteine using cyclic voltammetry.

Main Results:

  • Successfully synthesized FL-Au NPs using glucose oxidase.
  • Demonstrated selective detection of cysteine via fluorescence quenching and enhancement.
  • Observed visual color changes indicating cysteine presence.
  • Achieved electrochemical detection of cysteine with cyclic voltammetry.
  • Showcased the ability to tune sensitivity for cysteine detection across a wide pH and concentration range.

Conclusions:

  • FL-Au NPs synthesized with glucose oxidase provide a versatile platform for cysteine detection.
  • The multimodal sensing strategy enables robust and selective cysteine quantification.
  • The developed method offers tunable sensitivity, making it adaptable for various analytical needs.