Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Amperometry: Overview01:10

Amperometry: Overview

2.0K
Amperometry is a technique commonly used to measure the concentration of specific analytes in a solution by monitoring the electric current generated during an electrochemical reaction. It involves applying a constant potential between a working electrode and a reference electrode to measure the resulting current, which is proportional to the concentration of the analyte. The Clark oxygen electrode operates based on this principle of amperometry. It consists of a cathode and an anode enclosed...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Insulin Sensitivity and Beta Cell Function With Macupatide Alone or With Dulaglutide in Type 2 Diabetes: A Phase 1b, Randomised Controlled Trial.

Diabetes, obesity & metabolism·2026
Same author

Safety, Tolerability, Pharmacokinetics, and Pharmacodynamics of Petrelintide for Weight Management: Two Randomized, Controlled Phase 1 Trials.

Diabetes, obesity & metabolism·2026
Same author

Personal viewpoint: Risk stratification and counseling of living kidney donor candidates with prediabetes.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2026
Same author

Characterisation of steady-state pharmacokinetics and glucodynamics of once-weekly insulin efsitora alfa in individuals with type 2 diabetes.

Diabetes, obesity & metabolism·2026
Same author

Heart failure therapy in patients with advanced cancer receiving specialized palliative care (EMPATICC trial).

European heart journal·2025
Same author

Randomized investigation of heart failure therapy in patients with advanced cancer at risk of cardiac wasting: Rationale and design of the EMPATICC trial.

European journal of heart failure·2025

Related Experiment Video

Updated: Mar 9, 2026

Author Spotlight: Investigating the Blood Glucose Homeostasis in Murine Brain Using a Cost-Effective Hyperglycemic And Hypoglycemic Clamp Technique
07:35

Author Spotlight: Investigating the Blood Glucose Homeostasis in Murine Brain Using a Cost-Effective Hyperglycemic And Hypoglycemic Clamp Technique

Published on: January 26, 2024

2.3K

Improved Algorithm for Automated Glucose Clamps.

Mareike Kuhlenkötter1, Tim Heise1, Carsten Benesch1

  • 1Profil, Neuss, Germany .

Diabetes Technology & Therapeutics
|December 23, 2016
PubMed
Summary
This summary is machine-generated.

An improved algorithm significantly reduced blood glucose and glucose infusion rate variability in automated glucose clamps. This enhancement improves clamp quality without majorly impacting other key parameters.

Keywords:
Clamp qualityClampArtControl deviationGlucose clampPharmacodynamicsType 1 diabetes

More Related Videos

Hyperinsulinemic-Euglycemic Clamp in the Conscious Rat
11:12

Hyperinsulinemic-Euglycemic Clamp in the Conscious Rat

Published on: February 7, 2011

54.5K
Hyperinsulinemic-euglycemic Clamps in Conscious, Unrestrained Mice
11:10

Hyperinsulinemic-euglycemic Clamps in Conscious, Unrestrained Mice

Published on: November 16, 2011

96.0K

Related Experiment Videos

Last Updated: Mar 9, 2026

Author Spotlight: Investigating the Blood Glucose Homeostasis in Murine Brain Using a Cost-Effective Hyperglycemic And Hypoglycemic Clamp Technique
07:35

Author Spotlight: Investigating the Blood Glucose Homeostasis in Murine Brain Using a Cost-Effective Hyperglycemic And Hypoglycemic Clamp Technique

Published on: January 26, 2024

2.3K
Hyperinsulinemic-Euglycemic Clamp in the Conscious Rat
11:12

Hyperinsulinemic-Euglycemic Clamp in the Conscious Rat

Published on: February 7, 2011

54.5K
Hyperinsulinemic-euglycemic Clamps in Conscious, Unrestrained Mice
11:10

Hyperinsulinemic-euglycemic Clamps in Conscious, Unrestrained Mice

Published on: November 16, 2011

96.0K

Area of Science:

  • Metabolic research
  • Biomedical engineering
  • Clinical diagnostics

Background:

  • Automated glucose clamps maintain stable blood glucose (BG) levels using variable glucose infusion rates (GIRs).
  • High-quality clamps require minimal BG and GIR variability.
  • Existing algorithms, like the Biostator, can exhibit oscillations in BG and GIR.

Purpose of the Study:

  • To reduce oscillations in both BG and GIR for improved glucose clamp quality.
  • To implement and validate an improved algorithm in the ClampArt device.

Main Methods:

  • Numerical simulations (in silico) of glucose clamps to refine the Biostator algorithm.
  • In vitro experiments using the ClampArt device with a glucose-water model.
  • In vivo studies, including a pilot and a larger clinical trial, comparing the original and optimized algorithms.

Main Results:

  • The improved algorithm successfully reduced BG and GIR oscillations across in silico, in vitro, and in vivo experiments.
  • Clinical study showed a significant reduction in the coefficient of variation (CV) for BG (6.0% to 4.2%) and GIR (60.7% to 43.5%).
  • Other clamp quality parameters, such as median deviation from target and utility, remained largely unaffected.

Conclusions:

  • The optimized algorithm effectively reduces BG and GIR oscillations in glucose clamp studies.
  • This improvement enhances clamp quality without substantially altering other critical parameters.
  • The optimized algorithm is now implemented in the ClampArt device for future research.