Mesenchymal stem cells from reaming material possess high osteogenic potential and react sensitively to bone...

Area of Science:

• Orthopedics
• Regenerative Medicine
• Stem Cell Biology

Background:

• Reamer-Irrigator-Aspirator (RIA) system provides femoral bone material for non-union treatment.
• Bone morphogenetic protein-7 (BMP-7) is used with autogenous bone grafts.
• In vivo evidence comparing BMP-7 effects on RIA-derived MSCs (RIA-MSCs) and iliac crest bone marrow MSCs (BMSCs) is lacking.

Purpose of the Study:

• To compare the osteogenic potential of RIA-MSCs and BMSCs stimulated with BMP-7 in vivo.
• To investigate the dose-dependent response of RIA-MSCs and BMSCs to BMP-7.

Main Methods:

• Mesenchymal stem cells (MSCs) were isolated from 11 RIA and iliac crest bone marrow donors.
• Constructs of MSCs, β-tricalcium phosphate, and two BMP-7 concentrations (0.1 µg/mL, 1 µg/mL) were implanted in mice for 8 weeks.
• Bone formation was assessed quantitatively and qualitatively.

Main Results:

• All cell populations confirmed as MSCs.
• Bone formation observed in all constructs.
• RIA-MSCs showed significantly higher bone formation than BMSCs, especially without or with 0.1 µg/mL BMP-7.
• RIA-MSCs demonstrated increased bone formation with 0.1 µg/mL BMP-7, but not higher concentrations.
• BMSCs showed increased bone formation with both 0.1 µg/mL and 1 µg/mL BMP-7.

Conclusions:

• RIA material yields MSCs with significant osteogenic potential.
• BMP-7 stimulation enhances MSC osteogenic potential.
• RIA-MSCs exhibit greater sensitivity to BMP-7 stimulation compared to BMSCs.