Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

3D Bioprinting of Bio-orthogonally Cross-Linked Microporous Scaffolds Using Monodisperse Hydrogel Microparticles Enables Mechanotransduction Analysis.

Analytical chemistry·2026
Same author

Integrating appreciative education with AI-assisted oral training for sustainable EFL learning: a study on speaking anxiety and oral proficiency.

Frontiers in psychology·2026
Same author

Conformational Dynamics-Driven Engineering of Nanobody Biosensor for Enhanced Detection of Ochratoxin A.

Journal of agricultural and food chemistry·2026
Same author

A Nomogram for Predicting the Risk of Spinal Anesthesia-Induced Hypotension in Older Patients.

Diagnostics (Basel, Switzerland)·2026
Same author

Integrated metagenomic and metabolomic analysis reveals regional style differences in Maotai-flavour Baijiu.

Current research in microbial sciences·2026
Same author

An Improved Diffusion Model for Generating Images of a Single Category of Food on a Small Dataset.

Foods (Basel, Switzerland)·2026

Related Experiment Video

Updated: Mar 9, 2026

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

9.1K

Centrifugal micro-channel array droplet generation for highly parallel digital PCR.

Zitian Chen1, Peiyu Liao1, Fangli Zhang1

  • 1Biodynamic Optical Imaging Center (BIOPIC), Beijing Advanced Innovation Center for Genomics (ICG), Peking-Tsinghua Center for Life Sciences, and College of Engineering, Peking University, Beijing, China. yanyi@pku.edu.cn.

Lab on a Chip
|December 24, 2016
PubMed
Summary

We developed a novel micro-channel array (MiCA) technology for efficient, high-throughput production of stable, monodisperse emulsion droplets using a standard centrifuge. This cost-effective method simplifies digital PCR (dPCR) and other bioanalytical reactions.

More Related Videos

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

14.1K
Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics
09:05

Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics

Published on: June 29, 2020

5.6K

Related Experiment Videos

Last Updated: Mar 9, 2026

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

9.1K
Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

14.1K
Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics
09:05

Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics

Published on: June 29, 2020

5.6K

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Microfluidics

Background:

  • Stable water-in-oil emulsions are critical for droplet-based microreactors in digital PCR (dPCR) and other bioanalytical assays.
  • Conventional methods often require complex microfluidic devices and precise control systems, limiting accessibility and increasing costs.

Purpose of the Study:

  • To develop a novel, cost-effective technology for producing monodisperse emulsion droplets with high efficiency and throughput.
  • To demonstrate the utility of this method in enabling high-dynamic-range dPCR reactions.

Main Methods:

  • Development of a micro-channel array (MiCA) integrated with a bench-top centrifuge.
  • Dispersion of an aqueous phase into monodisperse droplet jets via centrifugal spinning through the MiCA.
  • Submersion of droplets into an oil phase to form a stable water-in-oil emulsion.

Main Results:

  • Successfully produced monodisperse emulsion droplets with high efficiency and throughput.
  • Demonstrated stable emulsion formation suitable for dPCR applications.
  • Achieved high dynamic range in dPCR reactions using the MiCA approach.

Conclusions:

  • The MiCA technology offers a cost-effective, robust, and user-friendly alternative for picoliter droplet generation.
  • This method eliminates the need for complex microfluidic systems, reduces material loss, and minimizes cross-contamination.
  • MiCA technology simplifies and enhances droplet-based bioanalytical reactions using conventional laboratory equipment.