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Related Concept Videos

Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

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The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
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Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
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Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis

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sm"FISH"ing for Hedgehog.

Michael L Drummond1, Scott X Atwood2

  • 1Department of Developmental and Cell Biology, University of California, Irvine, Irvine, California, USA.

The Journal of Investigative Dermatology
|December 25, 2016
PubMed
Summary
This summary is machine-generated.

Patched receptors regulate Hedgehog signaling, crucial for skin and hair follicle development. Quantitative analysis shows these receptors form a signaling gradient that may guide hair follicle development.

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Dermatology

Background:

  • Patched (Ptch) receptors are key negative regulators of the Hedgehog signaling pathway.
  • Loss of Ptch1 is linked to basal cell carcinoma, while combined loss of Ptch1 and Ptch2 affects skin and hair follicle development.

Purpose of the Study:

  • To quantitatively investigate the role of Ptch receptors in establishing Hedgehog signaling gradients.
  • To determine how these gradients may specify hair follicle development.

Main Methods:

  • Utilized single molecule fluorescent in situ hybridization (smFISH).
  • Performed quantitative analysis of Ptch receptor expression and Hedgehog signaling activity.

Main Results:

  • Demonstrated that Ptch receptors quantitatively establish a Hedgehog signaling gradient.
  • Provided evidence that this gradient is involved in specifying hair follicle development.

Conclusions:

  • Ptch receptors play a critical role in creating spatial signaling gradients essential for developmental processes.
  • The findings offer new insights into the molecular mechanisms underlying hair follicle morphogenesis.