[The fluorometric method for analysis of some acid drugs]

By using salicylate as a working drug, pyronine was chosen from 31 dyes to form drug-dye complex. In Na2HPO4 solution, salicylate reacts with pyronine to form a complex which is soluble in chloroform. Pyronine fluoresces strongly at lambda ex 542 nm and lambda cm 566 nm and the intensity of pyronine in complex is proportional to the amount of salicylate in the complex with detection limit of 5 ng/ml. By using this method, suberogorgin, flufenamic acid, mechlofenamic acid, mefenamic acid, sodium tauroglycocholate and trichloroacetic acid may be determined with the sensitivity of 10 approximately 50 ng/ml. Salicylate in blood was determined by this method with the sensitivity of 10 ng/ml blood. Good recovery and precision were obtained.