Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

"A chromosome-level reference genome for the colonial marine hydrozoan Podocoryna americana".

G3 (Bethesda, Md.)·2026
Same author

A chromosome-level genome assembly for the colonial marine hydrozoan <i>Podocoryna americana</i>.

bioRxiv : the preprint server for biology·2026
Same author

Public Health Impact and Cost-Effectiveness of Revising the Adolescent Meningococcal Vaccine Schedule in the United States.

PharmacoEconomics·2026
Same author

A Chromosome-level Assembly and Functional Genomic Resources for the Model Annelid Capitella teleta.

Genome biology and evolution·2026
Same author

<b>Gut bacterial</b> <i><b>O</b></i><b>-demethylation modulates systemic exposure to oral etoposide</b>.

Gut microbes·2026
Same author

<i>Fusobacterium nucleatum</i> produces previously unappreciated AHR-activating metabolites and promotes CRC cell proliferation via the AHR-TERT axis.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Mar 9, 2026

Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells
10:05

Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells

Published on: September 30, 2018

11.7K

Single cell transcriptomic profiling of mouse pancreatic progenitors.

Diana E Stanescu1,2, Reynold Yu2,3, Kyoung-Jae Won2,3

  • 1Division of Endocrinology and Diabetes, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania.

Physiological Genomics
|December 25, 2016
PubMed
Summary

Researchers identified Slc38a5 as a novel marker for early pancreatic alpha-cell precursors. This gene is expressed in immature alpha-cell progenitors but not in mature cells, aiding lineage studies.

Keywords:
alpha cell markerpancreas developmentsingle cell RNA-Seq

More Related Videos

In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas
09:31

In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas

Published on: June 10, 2016

9.9K
Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro
09:33

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro

Published on: June 2, 2018

9.5K

Related Experiment Videos

Last Updated: Mar 9, 2026

Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells
10:05

Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells

Published on: September 30, 2018

11.7K
In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas
09:31

In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas

Published on: June 10, 2016

9.9K
Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro
09:33

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro

Published on: June 2, 2018

9.5K

Area of Science:

  • Developmental biology
  • Endocrinology
  • Genomics

Background:

  • Developing pancreatic epithelium is heterogeneous.
  • Low abundance of endocrine progenitors limits traditional expression studies.

Purpose of the Study:

  • Identify genes characterizing early developmental tissues with multiple progenitor lineages.
  • Characterize alpha-cell precursors in embryonic pancreas.

Main Methods:

  • Single-cell RNA sequencing (scRNA-Seq) of embryonic day 13.5 mouse pancreata.
  • Integrative analysis with single-cell data from mature pancreas.
  • Immunofluorescence analysis of SLC38A5 expression.

Main Results:

  • Identified subpopulations with macrophage, endothelial, and novel pancreatic progenitor markers.
  • Found potential alpha-cell precursors expressing glucagon (Gcg) at embryonic day 13.5.
  • Discovered Slc38a5 as a characteristic gene in alpha-cell precursors, absent in mature alpha-cells.
  • Observed SLC38A5 expression in pancreatic progenitors, including NEUROG3+ and MAFB+ cells, and GCG+ cells.

Conclusions:

  • Slc38a5 is an early marker for alpha-cell lineage commitment.
  • SLC38A5 expression declines during late gestation and is absent in adult islets.
  • This study provides new markers for understanding pancreatic development.