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Mutants of luminous bacteria selected for bioluminescent toxicity tests
A Wagner1, U Winkler, P Lümmen
1Lehrstuhl f. Biologie d. Mikroorganismen, Ruhr-Universität, Bochum, FRG.
Journal of Bioluminescence and Chemiluminescence
|July 1, 1989
Abstract:
Mutants of the luminescent bacterial strain NRRL B-11177 were isolated with pleiotropic hypersensitivity towards hydrophobic antimicrobial agents. SDS-PAGE analyses of outer membrane proteins and lipopolysaccharides revealed that the outer membrane structure of the ahs-mutants was altered. QSAR analysis showed that the inhibitory effect of chloro-substituted phenols on bioluminescence of the ahs-mutants depended on their hydrophobicity. The effect of chlorinated phenols and detergents on bioluminescence was increased in the ahs-mutants. The potential use of these mutants in bioluminescent toxicity tests was discussed.