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Updated: Mar 9, 2026
Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue
Published on: June 6, 2016
Comparative phenotypic transcriptional characterization of human full-term placenta-derived mesenchymal stromal cells
Melanie L Hart1, Marvin Kaupp2, Juliane Brun2
1University of Freiburg, Department of Orthopedics and Trauma Surgery, Germany; Clinical Research Group KFO 273, Department of Urology, University of Tübingen, Germany.
Placenta-derived mesenchymal stromal cells (pMSCs) are a very attractive source of MSCs. In this short report we evaluated the expression of phenotypic markers from fetal and maternal pMSCs after exposure to myogenic medium commonly used to differentiate bone marrow MSCs (bmMSCs) to smooth muscle-like cells (SMCs). In order to reveal differences between these different MSC sources, cells were expanded and differentiated to elucidate whether this differentiation protocol facilitated efficient differentiation of SMCs from human pMSCs. We report that TGF-β1, PDGF and ascorbic acid is not sufficient to produce SMCs from pMSCs.
Placenta-derived mesenchymal stromal cells (pMSCs) are a very attractive source of MSCs. In this short report we evaluated the expression of phenotypic markers from fetal and maternal pMSCs after exposure to myogenic medium commonly used to differentiate bone marrow MSCs (bmMSCs) to smooth muscle-like cells (SMCs). In order to reveal differences between these different MSC sources, cells were expanded and differentiated to elucidate whether this differentiation protocol facilitated efficient differentiation of SMCs from human pMSCs. We report that TGF-β1, PDGF and ascorbic acid is not sufficient to produce SMCs from pMSCs.
