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Updated: Mar 9, 2026

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples
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Purification of a Human Prostate Specific Antigen.

M C Wang1, L A Valenzuela1, G P Murphy1

  • 1Department of Diagnostic Immunology Research and Biochemistry, Roswell Park Memorial Institute, Buffalo, New York.

The Journal of Urology
|December 26, 2016
PubMed
Summary

Researchers identified a new prostate-specific antigen (PSA) in human prostate tissues using rabbit antiserum. This novel PSA is present in normal, benign hypertrophic, and malignant prostates, distinct from prostatic acid phosphatase.

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Area of Science:

  • Urology
  • Immunology
  • Biochemistry

Background:

  • Prostate-specific antigen (PSA) is a key biomarker in prostate health.
  • Existing biomarkers like prostatic acid phosphatase have limitations.
  • The need for novel, specific prostate antigens is critical for diagnostics.

Purpose of the Study:

  • To identify and characterize a novel prostate-specific antigen (PSA) distinct from prostatic acid phosphatase.
  • To develop an antiserum for detecting this new PSA.
  • To purify the identified PSA to homogeneity.

Main Methods:

  • Generation of rabbit antiserum against normal human prostatic tissue extract.
  • Immunoprecipitation techniques to detect PSA.
  • Purification of PSA using polyacrylamide gel electrophoresis and isoelectric focusing.

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Main Results:

  • Antiserum successfully identified antibodies to a prostate tissue-specific antigen.
  • The antigen was detected in normal, benign hypertrophic, and malignant prostatic tissues.
  • The purified antigen exhibited a single protein band, confirming homogeneity and uniqueness from prostatic acid phosphatase.

Conclusions:

  • First demonstration of the purification of a prostate-specific antigen not representing prostatic acid phosphatase.
  • This novel PSA holds potential as a diagnostic marker for various prostate conditions.
  • Further research is warranted to explore its clinical utility.