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Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
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Imaging In focus: Reflected light imaging: Techniques and applications.

Emily J Guggenheim1, Iseult Lynch2, Joshua Z Rappoport3

  • 1Physical Science of Imaging in the Biomedical Sciences (PSIBS) Doctoral Training Centre (DTC), Birmingham, Edgbaston, UK; School of Geography, Earth and Environmental Sciences, Birmingham, Edgbaston, UK.

The International Journal of Biochemistry & Cell Biology
|December 26, 2016
PubMed
Summary
This summary is machine-generated.

Reflectance imaging uses scattered light to create images, offering non-destructive in vivo diagnostics and detailed study of nanomaterials. Advanced techniques provide superresolution and label-free investigation of cellular processes.

Keywords:
FreeLabelMicroscopyNanoparticlesReflectance

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Area of Science:

  • Biomedical Optics
  • Nanotechnology Imaging
  • In Vivo Diagnostics

Background:

  • Reflectance imaging detects back-scattered light from sample features.
  • Various configurations exist, including confocal, interferometry, and total internal reflectance.
  • Crucial for nanomaterial safety and understanding.

Purpose of the Study:

  • Highlight the diverse applications of reflectance imaging.
  • Emphasize its role in non-destructive in vivo diagnostics.
  • Showcase its utility in nanomaterial research and cellular studies.

Main Methods:

  • Utilizes diverse configurations like confocal, structured illumination, and interferometry.
  • Employs near-coverslip techniques such as reflectance interferometry and total internal reflection.
  • Enables superresolution imaging for enhanced spatial resolution.

Main Results:

  • Provides indispensable data for nanomaterial safety and characterization.
  • Facilitates non-destructive in vivo diagnostic strategies, potentially replacing biopsies.
  • Offers label-free investigation of cell-surface interactions, motility, and vesicle trafficking.
  • Achieves superresolution for increased imaging detail.

Conclusions:

  • Reflectance imaging is a versatile tool with significant biomedical applications.
  • Its non-destructive and label-free capabilities offer advanced diagnostic and research potential.
  • Superresolution advancements further expand its utility in biological and material sciences.