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A new technique for segmental jejunal perfusion in man.
L Knutson1, B Odlind, R Hällgren
1Department of Surgery, University Hospital, Uppsala, Sweden.
The American Journal of Gastroenterology
|October 1, 1989
Summary
A new multichannel tube accurately measures intestinal secretion of substances like albumin and histamine. This safe and rapid technique provides reliable biochemical insights into small intestine processes.
Area of Science:
- Gastroenterology
- Physiology
- Biochemistry
Background:
- Accurate measurement of endogenous substances in the small intestine is crucial for understanding gastrointestinal physiology.
- Existing methods may face challenges with contamination and dilution, affecting data reliability.
Purpose of the Study:
- To develop and validate a novel multichannel tube for measuring endogenous substance secretion in a defined segment of the small intestine.
- To assess the safety, efficacy, and reproducibility of this new technique.
Main Methods:
- A multichannel tube with occluding balloons was developed and inserted into the proximal jejunum under fluoroscopic guidance.
- The system was designed to prevent perfusate contamination and minimize dilution of intestinal secretions.
- Secretion rates of albumin, beta 2-microglobulin, hyaluronan, and histamine were measured in healthy controls.
Main Results:
- The procedure had a 90% success rate, with high recovery (>95%) of perfusion fluid and volume marker (14C-PEG 4000).
- Stable mean secretion rates were observed for albumin, beta 2-microglobulin, hyaluronan, and histamine in healthy controls.
- Analysis of substance concentrations relative to serum levels provided insights into passive leakage and active transport mechanisms.
Conclusions:
- The developed multichannel tube technique is simple, rapid, atraumatic, safe, and reproducible.
- This method accurately reflects biochemical processes in the small intestine, offering a valuable tool for research and diagnostics.