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Carbohydrate analysis on hybrid poly(dimethylsiloxane)/glass chips dynamically coated with ionic complementary

Nan Li1, Xiaoman Hai1, Xiaoling Yu1

  • 1School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, China.

Journal of Chromatography. A
|December 27, 2016
PubMed
Summary

A novel peptide coating method enhances carbohydrate separation in microfluidic chips. This technique minimizes unwanted adsorption and electroosmotic flow (EOF) for improved analytical results.

Keywords:
Dynamic coatingsGlycan extractsHybrid poly(dimethylsiloxane)/glass microchannelIonic complementary peptideMaltodextrin ladder

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Materials Science

Background:

  • Microfluidic chips are powerful analytical tools.
  • Nonspecific adsorption and electroosmotic flow (EOF) can hinder performance.
  • Efficient surface modification is crucial for high-performance microfluidic separations.

Purpose of the Study:

  • To develop a facile and efficient dynamic coating method for hybrid poly(dimethylsiloxane)/glass microfluidic channels.
  • To improve the separation of 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled carbohydrates.
  • To suppress nonspecific analyte adsorption and minimize EOF.

Main Methods:

  • A self-assembling ionic complementary peptide (EAK16-II) was used for dynamic surface coating.
  • Systematic investigation of EAK16-II concentration, running buffer, pH, and field strength.
  • Separation of APTS-labeled carbohydrates, including maltodextrin ladder and glycans from complex biological extracts.

Main Results:

  • EAK16-II formed a complete coating layer on both PDMS and glass surfaces.
  • Achieved high-performance separation with over 450,000 theoretical plates per meter.
  • Demonstrated rapid and reproducible separations with relative standard deviations (RSD) < 3.2% for migration times.

Conclusions:

  • The EAK16-II dynamic coating method is effective for high-performance carbohydrate analysis in microfluidic devices.
  • This approach minimizes EOF and nonspecific adsorption, broadening microfluidic applications in complex biological assays.
  • The method offers a versatile solution for surface modification in microfluidic chips made from various substrates.