Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrative venomics of the coral snake Micrurus elegans: composition, toxicity, and neutralization by antivenom.

Biochimie·2026
Same author

Structural and pharmacological analysis of a PLA<sub>2</sub>-like toxin in complex with the sPLA<sub>2</sub> inhibitor AZD2716: Comparisons to varespladib.

Biochimie·2026
Same author

InstaNovo enables diffusion-powered de novo peptide sequencing in large-scale proteomics experiments.

Nature machine intelligence·2026
Same author

New Insights into the Bioenergetic and Immunomodulatory Properties of Phospholipases A<sub>2</sub> from <i>Bothrops diporus</i> Venom.

Toxins·2026
Same author

First characterization of the venom of the endemic coral snake Micrurus camilae (Serpentes: Elapidae) from Colombia: Proteome, toxic activities, immunorecognition, and neutralization by antivenoms.

PLoS neglected tropical diseases·2026
Same author

Pharmacological inhibition of myosin reduces the cytotoxic effect of a Lys49 PLA<sub>2</sub>-like myotoxin on human and rat cardiac muscle.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026

Related Experiment Video

Updated: Mar 9, 2026

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases
06:30

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases

Published on: August 27, 2021

3.7K

Pitfalls to avoid when using phage display for snake toxins.

Andreas Hougaard Laustsen1, Line Præst Lauridsen2, Bruno Lomonte3

  • 1Department of Biotechnology and Biomedicine, Technical University of Denmark, Denmark; Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark.

Toxicon : Official Journal of the International Society on Toxinology
|December 27, 2016
PubMed
Summary
This summary is machine-generated.

Phage display technology offers a promising, modern approach to developing antivenoms, overcoming limitations of traditional animal-derived therapies. This study addresses key challenges in phage display selection for animal toxins to improve antitoxin development.

Keywords:
Amber codonsAntibody technologyBiotinylationClone pickingNext generation antivenomPhage displayRecombinant antivenom

More Related Videos

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
12:36

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Published on: February 16, 2014

35.1K
High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli
12:16

High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli

Published on: July 30, 2014

24.9K

Related Experiment Videos

Last Updated: Mar 9, 2026

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases
06:30

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases

Published on: August 27, 2021

3.7K
A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
12:36

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Published on: February 16, 2014

35.1K
High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli
12:16

High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli

Published on: July 30, 2014

24.9K

Area of Science:

  • Biotechnology and Immunology
  • Antivenom Development
  • Phage Display Technology

Background:

  • Current antivenoms derived from hyper-immunized animals cause side effects and are costly.
  • Need for biotechnological innovation in envenoming therapies.
  • Phage display offers a high-throughput method for discovering antibody-based antitoxins.

Purpose of the Study:

  • To explore phage display technology as a modern alternative for antivenom production.
  • To identify and address critical challenges encountered during phage display selection using animal toxins.
  • To provide solutions for researchers to avoid common pitfalls in phage display experiments.

Main Methods:

  • Utilized phage display technology for the discovery of antibody fragments against animal toxins.
  • Investigated challenges related to antigen biotinylation.
  • Addressed issues with clone picking and the presence of amber codons in antibody fragment structures.

Main Results:

  • Identified specific challenges in biotinylation of antigens for phage display.
  • Encountered and analyzed difficulties in clone picking processes.
  • Observed the impact of amber codons on antibody fragment display and function.

Conclusions:

  • Phage display technology holds significant potential for advancing antivenom development.
  • Overcoming technical challenges in antigen preparation, clone selection, and library construction is crucial for success.
  • Sharing solutions to these pitfalls can accelerate the development of next-generation biologics for envenoming therapies.