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Volumetric optical mapping in early embryonic hearts using light-sheet microscopy.

Pei Ma1, Dennis C Chan2, Shi Gu2

  • 1Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio 44106, USA; Shanghai Key Laboratory of Modern Optical Systems, College of Optical-Electrical and Computer Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.

Biomedical Optics Express
|December 27, 2016
PubMed
Summary

Volumetric optical mapping (OM) overcomes limitations of 2D imaging for embryonic cardiac electrophysiology. This new method provides complete, orientation-independent, four-dimensional maps of transmembrane potential in quail hearts.

Keywords:
(110.0110) Imaging systems(170.2520) Fluorescence microscopy(170.2655) Functional monitoring and imaging(170.3880) Medical and biological imaging(170.6900) Three-dimensional microscopy

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Area of Science:

  • Developmental biology
  • Cardiovascular research
  • Biophysics

Background:

  • Optical mapping (OM) is crucial for studying embryonic cardiac electrophysiology.
  • Conventional OM has limitations due to depth integration and 2D projection, complicating data interpretation.

Purpose of the Study:

  • To develop a novel volumetric OM technique to overcome the limitations of conventional OM.
  • To enable complete, orientation-independent, four-dimensional (4D) mapping of cardiac electrical activity.

Main Methods:

  • Utilized light-sheet microscopy for high-speed capture of optically sectioned slices.
  • Acquired voltage-sensitive fluorescence images from multiple planes of early embryonic quail hearts.
  • Employed optical pacing to control heart rate during data acquisition.

Main Results:

  • Demonstrated complete, orientation-independent 4D maps of transmembrane potential.
  • Successfully mapped electrical activity across entire early embryonic quail hearts.
  • Acquired volumetric OM data with controlled heart rate via optical pacing.

Conclusions:

  • Volumetric OM provides comprehensive and reliable data for embryonic cardiac electrophysiology.
  • This technique facilitates accurate comparisons between different samples.
  • Paves the way for future physiological measurements and precise heartbeat manipulation.