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Immunophenotypic and genotypic characterization of diffuse mixed non-Hodgkin's lymphomas
W E Katzin1, M D Linden, A J Fishleder
1Department of Laboratory Hematology, Cleveland Clinic Foundation, Ohio 44195-5138.
The American Journal of Pathology
|October 1, 1989
Summary
Diffuse, mixed small and large cell lymphomas (DML) are monoclonal B-cell lymphomas. These studies show DML comprises neoplastic B-cells and a non-neoplastic T-cell component, clarifying their lineage.
Area of Science:
- Hematology
- Oncology
- Immunopathology
Background:
- Diffuse, mixed small and large cell lymphomas (DML) represent a heterogeneous group of non-Hodgkin's lymphomas.
- Limited immunophenotypic and genotypic studies exist for DML, leaving their cellular lineage and clonality uncertain.
Purpose of the Study:
- To investigate the immunophenotype and genotype of DML.
- To determine the lineage and clonality of the neoplastic cells in DML.
Main Methods:
- Immunohistology using monoclonal antibodies on paraffin-embedded tissue.
- Frozen section immunohistology and cytospin immunocytology.
- Southern blotting/DNA hybridization for immunoglobulin and T cell receptor gene rearrangements.
Main Results:
- Immunohistology revealed large B lymphocytes in all 13 DML cases.
- Monoclonal B lymphocytes were supported by immunoglobulin light chain detection in 6 cases.
- The small cell component predominantly comprised T lymphocytes (12 of 13 cases).
- Immunoglobulin gene rearrangements were detected in 7 cases; T cell receptor gene rearrangements were not detected.
Conclusions:
- Diffuse, mixed small and large cell lymphomas (DML) are monoclonal lymphomas of B-cell origin.
- DML is characterized by a neoplastic B-cell population and a non-neoplastic T-cell infiltrate.