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Rapid detection of Streptococcus suis serotype 2 in weaned pigs
A Moreau1, R Higgins, M Bigras-Poulin
1Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, Qc, Canada.
American Journal of Veterinary Research
|October 1, 1989
Summary
A new method using precipitation zone diameter effectively screens for Streptococcus suis serotype 2 in weaned pigs. This technique offers high specificity and sensitivity for detecting the swine pathogen.
Area of Science:
- Veterinary Microbiology
- Swine Health Management
- Bacteriology
Background:
- Streptococcus suis serotype 2 is a significant swine pathogen.
- Accurate detection methods are crucial for controlling S. suis serotype 2 outbreaks in swine herds.
Purpose of the Study:
- To develop and validate a simple, reliable method for detecting Streptococcus suis serotype 2 in weaned pigs.
- To evaluate the efficacy of using precipitation zone diameter for S. suis serotype 2 screening.
Main Methods:
- Bacteriologic culture of nasal and tonsillar swabs from 1,716 weaned pigs across 49 sow herds.
- Detection of S. suis serotype 2 using selective agar and goat antiserum.
- Measurement of precipitation zone diameter and slide agglutination test for isolate identification.
Main Results:
- The precipitation zone diameter was significantly larger (P < 0.001) for S. suis serotype 2 isolates compared to others.
- Discriminant analysis classified 93.1% of isolates correctly using precipitation zone diameter.
- The method demonstrated high relative specificity (94.5%) and sensitivity (88.7%).
Conclusions:
- Quantitative measurement of precipitation zone diameter provides a simple and reliable screening technique for S. suis serotype 2 in weaned pigs.
- This method aids in efficient swine herd screening for S. suis serotype 2.
- Concurrent nasal and tonsillar sampling found S. suis serotype 2 in only 20.4% of carrier pigs.