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Updated: Mar 9, 2026

Pooled shRNA Library Screening to Identify Factors that Modulate a Drug Resistance Phenotype
Published on: June 17, 2022
[hnRNP K Contributes to Adriamycin Resistance in Acute Myeloid Leukemia through Regulating Autophagy]
Jin-Fang Zhang1, Xiao-Li Liu2, Yu-Deng Lin1
1Department of Paediatric Hematology and Oncology, Clinical Center of Tumor Therapy, Guangdong Provincial People Hospital, Guangdong Provincial Academy of Medical Sciences, Guangzhou 510080, Guangdong Province, China.
Objective:
To explore the role of heterogeneous nuclear ribonucleoprotein(hnRNP) K regulating autophagy in the drug resistance of acute myeloid leukemia, so as to provide a new molecular marker for treatment of leukemia.
Methods:
The relationship between the expression level of hnRNP K and the drug resistance of myeloid leukemia was verified by fluorescence quantitative PCR; the expression of autophagy related protein LC3I/ II was detected by Western blot after the hnRNP K was modulated by RNA interference technology; the sensitivity of leukemia cells to doxorubicin was analyzed before and after the expression of hnRNP K were modulatd.
Results:
The expression of hnRNP K and LC3I/II significantly increased in bone marrow nonremission patients and in drug resistant cell line, however, the expression of LC3I/ II decreased when the expression of hnRNP K were reduced, while the sensitivity of cells to adriamycin could be recovered.
Conclusion:
hnRNP K may be involved in the formation of adriamycin resistance in acute myeloid leukemia by regulating autophagy.
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