Novel impedimetric dopamine biosensor based on boronic acid functional polythiophene modified electrodes

Area of Science:

• Electrochemistry
• Biosensor Technology
• Analytical Chemistry

Background:

• Dopamine detection is crucial for diagnosing neurological disorders.
• Existing methods for dopamine detection can be complex and time-consuming.
• Development of sensitive and selective biosensors is an active area of research.

Purpose of the Study:

• To develop a new, simple, and first impedimetric biosensor for dopamine detection.
• To utilize 3-Thienyl boronic acid for creating a highly selective and stable sensing electrode.
• To evaluate the biosensor's performance in real-world samples.

Main Methods:

• Electrode preparation via electro-polymerization of 3-Thienyl boronic acid and Thiophene copolymer (P(TBA0.50Th0.50)).
• Characterization and optimization using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV).
• Testing reliability through application studies in non-diluted human urine samples.

Main Results:

• The biosensor exhibits high selectivity and stability due to strong dopamine-boronic acid interaction.
• Achieved a linear detection range of 7.8 to 125 μM with a detection limit of 0.3 μM.
• Demonstrated excellent selectivity and recovery in human urine samples with minimal interference.

Conclusions:

• The developed impedimetric biosensor based on 3-Thienyl boronic acid is a simple, rapid, and effective tool for dopamine detection.
• The P(TBA0.50Th0.50) electrode shows high reproducibility, stability, and selectivity.
• The biosensor's performance in real samples confirms its practical applicability.