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Calypso: a user-friendly web-server for mining and visualizing microbiome-environment interactions.

Martha Zakrzewski1, Carla Proietti1, Jonathan J Ellis2

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Summary
This summary is machine-generated.

Calypso is a user-friendly software for analyzing metagenomic and 16S rDNA data. It employs multivariate statistics to reveal intricate environment-microbiome relationships for researchers.

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Area of Science:

  • Microbiology
  • Bioinformatics
  • Computational Biology

Background:

  • Metagenomic and 16S rDNA sequencing generate complex datasets.
  • Analyzing these datasets requires specialized bioinformatics tools.
  • Identifying associations between environmental factors and microbial communities is crucial.

Purpose of the Study:

  • To introduce Calypso, an accessible online software suite.
  • To enable non-expert users to mine, interpret, and compare taxonomic data.
  • To facilitate the identification of environment-microbiome associations using multivariate statistics.

Main Methods:

  • Calypso utilizes a web-interface for user interaction.
  • The software is programmed in Java, PERL, and R.
  • It incorporates multivariate statistical approaches, including factor analysis, regression, and network analysis.

Main Results:

  • Calypso provides quantitative visualizations and statistical testing.
  • The software supports diversity estimates and supervised learning.
  • It enables comprehensive analysis of complex environment-microbiome interactions.

Conclusions:

  • Calypso empowers non-expert users in analyzing microbial community data.
  • The software offers a robust platform for exploring ecological associations.
  • Freely available for non-commercial use, Calypso enhances microbiome research accessibility.