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An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

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Published on: January 11, 2017

Transcript Polymorphism Rates in Soybean Seed Tissue Are Increased in a Single Transformant of Glycine max.

Kevin C Lambirth¹, Adam M Whaley², Jessica A Schlueter²

¹Department of Bioinformatics and Genomics, University of North Carolina at Charlotte, North Carolina Research Campus, Kannapolis, NC 28081, USA.

International Journal of Plant Genomics

|December 28, 2016

Summary

Transgenic soybean research reveals increased genetic variations, including single nucleotide polymorphisms (SNPs), in seed tissues. Despite these changes, the overall plant health and traits remained unaffected.

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Area of Science:

Agricultural Biotechnology
Molecular Genetics
Plant Breeding

Background:

Transgenic crops are used in agriculture and as bioreactors for pharmaceuticals.
Previous gene expression analysis identified distinct transgenic soybean events.

Purpose of the Study:

To investigate polymorphisms and sequence variations in the exomes of transgenic soybean events.
To analyze single nucleotide polymorphism (SNP) rates in transgenic soybean seed tissues.

Main Methods:

Exome sequencing of transgenic soybean events.
Analysis of polymorphisms and sequence variations in mRNA transcripts from seed tissue.

Main Results:

A specific transgenic soybean line showed significantly increased polymorphisms in seed mRNA transcripts.
Many identified polymorphisms were classified as gene expression modifiers.
This study is the first to investigate SNP rates in transgenic soybean seed tissues.

Conclusions:

Transgenesis can induce substantial, unanticipated changes in gene expression and nucleotide variation.
Identified SNPs can guide future breeding efforts for improved transgenic soybean crops.
Phenotypes and overall plant health were unaltered in the examined transgenic soybean plants.