Liposomal Spherical Nucleic Acids for Regulating Long Noncoding RNAs in the Nucleus
Anthony J Sprangers1, Liangliang Hao2, Resham J Banga3
1Department of Biomedical Engineering, International Institute for Nanotechnology, Northwestern University, Evanston, IL, 60208, USA.
Small (Weinheim an Der Bergstrasse, Germany)
|December 28, 2016
Summary
Researchers developed liposomal spherical nucleic acids (LSNAs) to target and reduce oncogenic long noncoding RNAs (lncRNAs) like Malat1 in cancer cells, showing therapeutic potential.
Area of Science:
- Molecular Biology
- Nanotechnology
- Oncology
Background:
- Long noncoding RNAs (lncRNAs) play critical roles in cellular development and cancer.
- The metastasis associated lung adenocarcinoma transcript 1 (Malat1) is an oncogenic lncRNA implicated in cancer metastasis.
Purpose of the Study:
- To investigate the use of spherical nucleic acids (SNAs) as a nanoparticle platform for regulating lncRNAs.
- To achieve targeted knockdown of the nuclear-retained Malat1 lncRNA using SNAs.
Main Methods:
- Development and utilization of liposomal spherical nucleic acid (LSNA) constructs.
- Exploration of antisense oligonucleotide delivery to the cell nucleus using LSNA.
- Assessment of Malat1 inhibition and downstream effects on messenger RNA (mRNA) expression.
Main Results:
- Successful delivery of antisense oligonucleotides to the nucleus via LSNA constructs.
- Demonstration of dose-dependent inhibition of Malat1 expression following LSNA treatment.
- Observation of consequent up-regulation of tumor suppressor mRNAs after Malat1 knockdown.
Conclusions:
- LSNAs represent an effective nanoparticle platform for regulating nuclear-retained lncRNAs.
- LSNA-based antisense strategies hold significant biologic and therapeutic potential for targeting oncogenic lncRNAs in cancer.
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