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Related Experiment Video

Updated: Mar 9, 2026

Monitoring Conformational Dynamics of Single Unmodified Proteins using Plasmonic Nanotweezers
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Light-Enhancing Plasmonic-Nanopore Biosensor for Superior Single-Molecule Detection.

Ossama N Assad1,2, Tal Gilboa1, Joshua Spitzberg1

  • 1Department of Biomedical Engineering, The Technion -Israel Institute of Technology, Haifa, 32000, Israel.

Advanced Materials (Deerfield Beach, Fla.)
|December 28, 2016
PubMed
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A novel plasmonic biosensor significantly reduces background noise and enhances fluorescence, enabling highly sensitive single-molecule detection. This technology achieves superior signal-to-background ratios for DNA sensing with minimal laser power.

Area of Science:

  • Nanotechnology
  • Biophysics
  • Analytical Chemistry

Background:

  • Background fluorescence in biosensing limits sensitivity and resolution.
  • Existing methods struggle with high signal-to-background ratios for single-molecule detection.
  • Low excitation laser intensities are desirable to minimize photobleaching and damage.

Purpose of the Study:

  • To develop a biosensor with enhanced fluorescence intensity and suppressed background.
  • To achieve a high signal-to-background ratio for single-molecule detection.
  • To enable high-temporal-bandwidth sensing of single DNA molecules.

Main Methods:

  • Fabrication of a stacked plasmonic nanowell-nanopore structure.
  • Utilizing plasmonic enhancement for fluorescence detection.
Keywords:
DNA sequencingfluorescence enhancementplasmonic nanostructuressingle-molecule sensingsolid-state nanopores

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  • Characterization of signal-to-background ratio and temporal bandwidth.

    • Main Results:

    • Demonstrated strong suppression of bulk background fluorescence.
    • Achieved over tenfold net enhancement in fluorescence intensity.
    • Obtained an extremely high signal-to-background ratio for single-molecule detection.
    • Maintained high temporal bandwidth suitable for single-DNA sensing at ultralow laser intensities.

    Conclusions:

    • The stacked plasmonic nanowell-nanopore biosensor offers a significant advancement in sensitive molecular detection.
    • The device enables robust single-molecule analysis with improved signal quality and reduced excitation power.
    • This technology has potential applications in various fields requiring high-sensitivity biomolecular detection.