Expression of Bruton's tyrosine kinase in B-cell neoplasms evaluated by flow cytometry
Natália Aydos Marcondes1,2, Flavo Beno Fernandes3, Ana Paula Alegretti4
1Programa de Pós Graduação em Medicina: Ciências Médicas, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos 2400, 2° andar, Porto Alegre, 90035-903, Brazil. nam_nati@yahoo.com.br.
Abstract:
Bruton's tyrosine kinase (BTK) is a cytoplasmatic protein that is part of the B-cell antigen receptor signaling pathway. Our aim was to evaluate the expression of BTK in B-cell neoplasms and compare it to normal B-cell lymphocytes. After surface staining with CD19 and CD45, flow cytometry staining for intracellular BTK was performed in leukemic or mature B-cells from bone marrow or peripheral blood samples. No differences in BTK expression were identified between groups, or in comparison to control samples, there was no association between BTK expression and the clinical variables evaluated. BTK expression in B-cell neoplasms was similar to that of normal B-cell lymphocytes.
