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[Intestinal microbial ecology and its modulation under the influence of immunodepressants]
Abstract:
Oral administration of immunodepressants such as imuran (purine analog) and batriden (gossypol derivative) for 3 months led to development of dysbacterioses in various sections of the rat gastrointestinal tract. The dysbacterioses differed in their levels and the pattern of the recovery process. As compared to batriden, imuran in a dose of 30 mg/kg body weight administered at the early observation periods (days 7, 14 and 30) induced more marked disorders in the intestine microecology. The imuran-induced dysbacteriosis was characterized by lower quantities of lactobacilli and bifidobacteria in the rat intestine. After the use of batriden the quantities of bifidobacteria, lactobacilli and bacteroides decreased. After the batriden use at the late observation periods (days 60 to 90) the ratio of anaerobes and lactobacilli to aerobes recovered at the background of increased quantities of Candida in all the intestine sections while the ratio of bacteroides recovered in the stomach. When immunity was suppressed by imuran the recovery period was characterized by normalization of the microflora composition in the distal sections and preservation of the contamination symptom in the proximal section which was evident from predominance of aerobes over anaerobes.
Insights
Oral administration of imuran and batriden caused gut dysbacteriosis in rats. Imuran significantly disrupted intestinal microecology, reducing beneficial bacteria more than batriden.
Area of Science:
- Microbiology
- Pharmacology
- Gastroenterology
Context:
- Immunosuppressive drugs can alter the gut microbiome.
- Understanding drug-induced dysbacteriosis is crucial for patient health.
- Rat models are used to study gastrointestinal tract changes.
Purpose:
- To investigate the effects of imuran and batriden on rat gut microbiota.
- To compare the dysbacteriosis patterns induced by these two immunosuppressants.
- To analyze the recovery process of the gut microecology after drug administration.
Summary:
- Oral administration of imuran (purine analog) and batriden (gossypol derivative) for 3 months induced dysbacteriosis in the rat gastrointestinal tract.
- Imuran (30 mg/kg) caused more severe early-stage intestinal microecology disorders, decreasing lactobacilli and bifidobacteria.
- Batriden reduced bifidobacteria, lactobacilli, and bacteroides; later recovery showed increased Candida with imuran, while batriden led to partial recovery.
Impact:
- Highlights the significant impact of common immunosuppressants on gut microbial balance.
- Provides insights into differential effects of imuran versus batriden on gut microbiota composition.
- Informs potential strategies for managing drug-induced gut dysbiosis and its long-term consequences.