Related Experiment Video

Updated: Mar 9, 2026

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia
09:52

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia

Published on: December 4, 2018

8.1K

Glycogen Synthase Kinase-3β (GSK-3β) and Nuclear Factor Kappa-B (NFKB) in Childhood Acute Lymphoblastic Leukemia

Cristian Fabian Layton Tovar1, Hugo Mendieta Zerón2, Maria Del Socorro Camarillo Romero3

  • 1School of Medicine, Autonomous University of the State of Mexico, Toluca, Mexico.

Advances in Clinical and Experimental Medicine : Official Organ Wroclaw Medical University
|December 29, 2016
PubMed

Insights

Glycogen synthase kinase 3beta (GSK-3β) and nuclear factor kappa beta (NFKB) may serve as prognostic biomarkers in pediatric acute lymphocytic leukemia (ALL). This study found significant differences in NFKB expression related to GSK-3β activity in bone marrow samples.

Area of Science:

  • Pediatric Hematology
  • Oncology Research
  • Molecular Biology

Background:

  • Acute lymphocytic leukemia (ALL) is a common childhood cancer.
  • Glycogen synthase kinase 3beta (GSK-3β) activity is linked to nuclear factor kappa beta (NFKB) expression in pediatric ALL.
  • Understanding these markers could improve prognostic assessments.

Purpose of the Study:

  • To investigate the prognostic significance of GSK-3β and NFKB in pediatric ALL.
  • To correlate GSK-3β activity and NFKB expression with disease characteristics.

Main Methods:

  • Observational study of 30 newly-diagnosed pediatric ALL patients.
  • Analysis of bone marrow and blood samples using immunohistochemistry for GSK-3β and qPCR for NFKB mRNA.
  • Classification of ALL cases into high and habitual risk groups.

Main Results:

  • NFKB relative expression showed significant differences based on GSK-3β immunohistochemistry results (p = 0.001 for positive vs. negative, p = 0.002 for weak-positive vs. negative).
  • Genetic abnormalities were identified in 23.33% of patients.
  • The study included patients aged 2-13 years, with a majority being male.

Conclusions:

  • GSK-3β shows potential as a prognostic biomarker in childhood ALL.
  • Further research is warranted to validate GSK-3β and NFKB as reliable prognostic indicators.
Abstract

Keywords:
GSK-3βNFKBacute lymphoblastic leukemiaprognostic

More Related Videos

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants07:38

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

882
Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

10.6K

Related Concept Videos

NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
10.2K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
10.8K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
13.5K
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
8.9K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.3K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.7K