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Three-Dimensional Ordered Antibody Arrays Through Self-Assembly of Antibody-Polymer Conjugates
Xue-Hui Dong1, Allie C Obermeyer1, Bradley D Olsen1
1Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
Abstract:
Three-dimensional (3D) ordered arrays of human immunoglobulin G (IgG) were fabricated using well-defined full-length antibody-polymer conjugates (APCs). The conjugates were prepared through a two-step sequential click approach with a combination of oxime ligation and strain promoted alkyne-azide cycloaddition. They were able to self-assemble into lamellar nanostructures with alternating IgG and poly(N-isopropylacrylamide) (PNIPAM) nanodomains. As a proof-of-concept, these materials were fabricated into thin films and their specific binding ability was tested. The nanostructure not only improves the packing density and the proper orientation of the IgG, but also provides nanochannels to facilitate substrate transport.
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