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Evaluation of procoagulant tissue factor expression in canine hemangiosarcoma cell lines
American Journal of Veterinary Research
|December 29, 2016
Summary
Canine hemangiosarcoma cells express tissue factor (TF), a procoagulant protein, in vitro. This TF expression contributes to thrombin generation, suggesting potential roles in hemostatic dysfunction in dogs with this cancer.
Area of Science:
- Veterinary Oncology
- Hemostasis and Thrombosis
- Molecular Biology
Background:
- Canine hemangiosarcoma is an aggressive cancer associated with hemostatic abnormalities.
- Tissue factor (TF) is a key initiator of the extrinsic coagulation pathway and can be expressed by tumor cells.
Purpose of the Study:
- To investigate the expression of procoagulant tissue factor (TF) by canine hemangiosarcoma cell lines in vitro.
- To assess the functional impact of TF expression on thrombin generation.
Main Methods:
- Cultured canine hemangiosarcoma cell lines and a nonneoplastic endothelial cell line.
- Quantitative real-time PCR for TF mRNA and flow cytometry for TF antigen.
- Calibrated automated thrombography to measure thrombin generation in various plasma conditions.
Main Results:
- All tested hemangiosarcoma cell lines expressed TF mRNA and antigen, with higher levels in specific aggressive lines (SB-HSA, Emma).
- Hemangiosarcoma cells induced significantly greater thrombin generation compared to control endothelial cells.
- TF-initiated thrombin generation was dependent on factors VII and X, and phosphatidylserine exposure.
Conclusions:
- Canine hemangiosarcoma cells express functional procoagulant tissue factor in vitro.
- TF expression by tumor cells may contribute to hemostatic dysfunction observed in dogs with hemangiosarcoma.
- Further research is warranted to explore TF as a potential biomarker for hemostatic complications.
