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Preparation and Identification of Monoclonal Antibody Against C1q/TNF-Related Protein 4

He Huang1, Xiaotong Wu1, Lulu Cao1

  • 1Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center , Beijing, China .

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
|December 29, 2016
PubMed

CTRP4 (C1q/TNF-related protein 4) is a secreted cytokine homologous to adiponectin, which plays an important role in immunity and metabolism. This study was designed to generate CTRP4 monoclonal antibodies (MAbs). Splenocytes extracted from mice immunized with prokaryotic protein were fused with myeloma cells Sp2/0 to generate hybridoma cells. Three hybridoma strains (16, 33, and 35) were chosen and their MAbs were purified. The specificity and affinity were identified by Western blot and enzyme-linked immunosorbent assay, whereas CTRP4 endogenous expression was identified by immunofluorescence staining. These MAbs could be useful tools for basic research and potential clinical application.

Keywords:
CTRP4affinitysubcellular location

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