Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Wick technique: reference method for implanted glucose sensors.

U Fischer1, R Ertle, K Rebrin

  • 1Central Institute of Diabetes Gerhardt Katsch, Karlsburg, German Democratic Republic.

Artificial Organs
|October 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Estimation of urea production rate with [(15)n(2)]urea and [(13)c]urea to measure catabolic rates in diabetes mellitus.

Isotopes in environmental and health studies·2011
Same author

Age-dependent differences in demographics, risk factors, co-morbidity, etiology, management, and clinical outcome of acute ischemic stroke.

Journal of neurology·2008
Same author

[X-ray mammography : calcifications, masses and architectural distortions].

Der Radiologe·2008
Same author

Inflammation in fish as seen from a morphological point of view with special reference to the vascular compartment.

Current pharmaceutical design·2008
Same author

Glioblastoma multiforme: the role of DSB repair between genotype and phenotype.

Oncogene·2007
Same author

Interaction with PI3-kinase contributes to the cytotoxic activity of apoptin.

Oncogene·2007
Same journal

Large-Eddy Simulation of the FDA Benchmark Blood Pump: Validation Against Experiments and Implications for Turbulent Flow Mechanisms.

Artificial organs·2026
Same journal

The Warm Revolution: A Meta-Analysis of DCD Versus DBD Liver Transplant Outcomes in the Normothermic Machine Perfusion Era.

Artificial organs·2026
Same journal

Toward Optimal Remote Monitoring in LVAD Recipients: Remaining Challenges Beyond Feasibility.

Artificial organs·2026
Same journal

Advancing Organ Preservation and Perfusion: Introducing the International Society of Organ Preservation and Perfusion Therapy (ISOPPT).

Artificial organs·2026
Same journal

Short Inter-Treatment Interval Treatment With Artificial Liver Support System Reduces 90-Day Transplant-Free Mortality in Patients With Hepatitis B Virus-Related Acute-On-Chronic Liver Failure: A Retrospective Observational Study.

Artificial organs·2026
Same journal

Extracorporeal Albumin Dialysis (OPAL) as Novel Therapeutic Bridging Option in Posthepatectomy Liver Failure.

Artificial organs·2026
See all related articles

This study introduces a novel wick technique for collecting subcutaneous interstitial fluid in dogs. This method provides a reliable reference for calibrating implanted glucose sensors, crucial for accurate diabetes management.

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Endocrinology

Background:

  • Accurate glucose monitoring is essential for managing diabetes.
  • Implanted glucose sensors require independent reference methods for calibration.
  • Subcutaneous interstitial fluid (ISF) is a key analyte compartment for glucose sensing.

Purpose of the Study:

  • To develop and validate a novel method for obtaining ISF specimens.
  • To establish a reference method for calibrating in situ implanted glucose sensors.
  • To assess the correlation between ISF glucose and circulating glycemia.

Main Methods:

  • An implanted wick technique using saline-impregnated cotton threads was employed in dogs.
  • Subcutaneous ISF was collected and analyzed for potassium, calcium, and hemoglobin.

Related Experiment Videos

  • Wick fluid glucose levels were compared to circulating glucose concentrations during steady-state and dynamic conditions (oral glucose tolerance test).

    • Main Results:

    • Wick fluid accurately reflected ISF solute concentrations after a 15-minute equilibration period.
    • Subcutaneous glucose levels closely matched circulating glycemia between 2 and 25 mmol/L.
    • The wick method demonstrated a distinct delay in reflecting rapid glucose fluctuations but paralleled slow changes.
    • Wick-based glucose measurements correlated with electrochemical sensor outputs.

    Conclusions:

    • The implanted wick technique provides a validated method for obtaining ISF for glucose analysis.
    • This technique serves as a viable in situ reference for calibrating and checking implanted glucose sensors.
    • The method holds potential for improving the accuracy and reliability of continuous glucose monitoring systems.