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Updated: Mar 9, 2026
A Guide to Modern Quantitative Fluorescent Western Blotting with Troubleshooting Strategies
Published on: November 20, 2014
Quantifying western blots: none more black
1BMC Biology, London, UK. graham.bell@biomedcentral.com.
Background:
Western blotting is among the most common techniques used in molecular biology and a simple way of assessing the presence or absence of a protein. It is also commonly used to compare protein levels in different conditions or in different tissues. This article illustrates some of the easy ways to arrive at a false conclusion when trying to quantify protein levels from western blots.
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