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Updated: Mar 9, 2026
A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
Published on: November 23, 2015
Interfacing antibody-based microarrays and digital holography enables label-free detection for loss of cell volume
Zahra El-Schich1, Emmy Nilsson1, Anna S Gerdtsson2
1Department of Biomedical Science, Health & Society, Malmö University, Malmö, Sweden; Department of Biomedical Science, Health & Society, Malmö University, Malmö, Sweden.
Background:
We introduce the combination of digital holographic microscopy (DHM) and antibody microarrays as a powerful tool to measure morphological changes in specifically antibody-captured cells. The aim of the study was to develop DHM for analysis of cell death of etoposide-treated suspension cells.
Results/Methodology:
We demonstrate that the cell number, mean area, thickness and volume were noninvasively measured by using DHM. The cell number was stable over time, but the two cell lines showed changes of cell area and cell irregularity after treatment. The cell volume in etoposide-treated cells was decreased, whereas untreated cells showed stable volume.
Conclusion:
Our results provide proof of concept for using DHM combined with antibody-based microarray technology for detecting morphological changes in captured cells.
